Kinetic model for simultaneous cell disruption and aqueous two-phase extraction
文献类型：期刊论文
| 作者 | Chang, B. S. ; Sue, Z. G. |
| 刊名 | Journal of Chemical Technology and Biotechnology
 |
| 出版日期 | 2006 |
| 期号 | 3页码:454-460 |
| 关键词 | disruption kinetics aqueous two-phase extraction glucose-6-phosphate dehydrogenase lactate dehydrogenase alcohol dehydrogenase yeast saccharomyces-cerevisiae bakers-yeast bead mill proteins systems release purification integration cutinase |
| 其他题名 | J. Chem. Technol. Biotechnol. |
| 中文摘要 | A process of simultaneous cell disruption and aqueous two-phase extraction demonstrated improved product yield and selectivity compared with the traditional process of cell disruption followed by extraction. Addition of aqueous two-phase components did not decrease the efficiency of cell disruption. Moreover, ADH, LDH and G6PDH recovery was enhanced in this new process with recovery ratios of 97%, 93% and 95%, respectively. Cell disruption kinetics were established based on a new mechanism that was different from traditional first-order kinetics, consisting of the release of intracellular proteins and enzymes, the denaturation and subsequent renaturation of these proteins that may be due to the protection of the aqueous two-phase components during the cell disruption process. To account for this, the kinetics parameters were calculated, and the experimental data were regressed. The resulting kinetic model could provide a better fit for the experimental data with a correlation ratio of 99% (for total protein), and led to insights into the differences between the new process and the traditional one. (c) 2005 Society of Chemical Industry |
| 原文出处 | |
| 语种 | 英语 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4002]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | Chang, B. S.,Sue, Z. G.. Kinetic model for simultaneous cell disruption and aqueous two-phase extraction[J]. Journal of Chemical Technology and Biotechnology,2006(3):454-460. |
| APA | Chang, B. S.,&Sue, Z. G..(2006).Kinetic model for simultaneous cell disruption and aqueous two-phase extraction.Journal of Chemical Technology and Biotechnology(3),454-460. |
| MLA | Chang, B. S.,et al."Kinetic model for simultaneous cell disruption and aqueous two-phase extraction".Journal of Chemical Technology and Biotechnology .3(2006):454-460. |
入库方式： OAI收割
来源：过程工程研究所
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