A macrokinetic and regulator model for myeloma cell culture based on metabolic balance of pathways
文献类型：期刊论文
| 作者 | Zhou, Feng; Bi, Jing-Xiu; Zeng, An-Ping; Yuan, Jing-Qi |
| 刊名 | PROCESS BIOCHEMISTRY
 |
| 出版日期 | 2006-10-01 |
| 卷号 | 41期号:10页码:2207-2217 |
| 关键词 | myeloma metabolic pathways stoichiometric model regulator model metabolic shift |
| ISSN号 | 1359-5113 |
| 其他题名 | Process Biochem. |
| 中文摘要 | A macrokinetic model is proposed to simulate the growth and metabolism of myeloma cell line in batch and fed-batch cultures. The model describes glycolysis, glutaminolysis, the TCA cycle, respiratory chain, formation of metabolites, as well as cell growth and death. In particular, metabolic shift in the glucose utilization is considered in response to the change of glycolysis rate during the cultivation. A closed loop regulator is introduced to approximate the induction of enzyme pool during lag phase after inoculation. Based on the model, the specific consumption rate of substrate, the specific growth rate, the specific acetyl CoA formation rate as well as the specific oxygen uptake rate are estimated. The specific substrate consumption rate and the specific growth rate are then coupled into a bioreactor model, which delivers the key variables, i.e., cell density, substrate and metabolite concentrations. Batch and fed-batch cultivations were used to validate the model. (c) 2006 Elsevier Ltd. All rights reserved. |
| 英文摘要 | A macrokinetic model is proposed to simulate the growth and metabolism of myeloma cell line in batch and fed-batch cultures. The model describes glycolysis, glutaminolysis, the TCA cycle, respiratory chain, formation of metabolites, as well as cell growth and death. In particular, metabolic shift in the glucose utilization is considered in response to the change of glycolysis rate during the cultivation. A closed loop regulator is introduced to approximate the induction of enzyme pool during lag phase after inoculation. Based on the model, the specific consumption rate of substrate, the specific growth rate, the specific acetyl CoA formation rate as well as the specific oxygen uptake rate are estimated. The specific substrate consumption rate and the specific growth rate are then coupled into a bioreactor model, which delivers the key variables, i.e., cell density, substrate and metabolite concentrations. Batch and fed-batch cultivations were used to validate the model. (c) 2006 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | INTRACELLULAR FLUX ANALYSIS ; STEADY-STATE MULTIPLICITY ; HYBRIDOMA CELLS ; ANTIBODY-PRODUCTION ; OXIDATIVE-PHOSPHORYLATION ; DYNAMIC-MODEL ; SACCHAROMYCES-CEREVISIAE ; ENERGY-METABOLISM ; STRUCTURED MODEL ; REACTION NETWORK |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000241066600015 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4008]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Dept Automat, Shanghai 200240, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 3.German Res Ctr Biotechnol, Biochem Engn Div, D-38124 Braunschweig, Germany |
| 推荐引用方式 GB/T 7714 | Zhou, Feng,Bi, Jing-Xiu,Zeng, An-Ping,et al. A macrokinetic and regulator model for myeloma cell culture based on metabolic balance of pathways[J]. PROCESS BIOCHEMISTRY,2006,41(10):2207-2217. |
| APA | Zhou, Feng,Bi, Jing-Xiu,Zeng, An-Ping,&Yuan, Jing-Qi.(2006).A macrokinetic and regulator model for myeloma cell culture based on metabolic balance of pathways.PROCESS BIOCHEMISTRY,41(10),2207-2217. |
| MLA | Zhou, Feng,et al."A macrokinetic and regulator model for myeloma cell culture based on metabolic balance of pathways".PROCESS BIOCHEMISTRY 41.10(2006):2207-2217. |
入库方式： OAI收割
来源：过程工程研究所
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