Mass spectrometric analysis of enzymatic digestion of denatured collagen for identification of collagen type
文献类型：期刊论文
| 作者 | Zhang, G; Sun, A; Li, W; Liu, T; Su, Z |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2006-05-12 |
| 卷号 | 1114期号:2页码:274-277 |
| 关键词 | collagen identification tryptic digestion mass spectrometry |
| ISSN号 | 0021-9673 |
| 其他题名 | J. Chromatogr. A |
| 中文摘要 | Collagen type II and I from bovine were thermally denatured and digested with trypsin. The digest mixture was analyzed with liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS). Peptides in the digest mixture were identified by mass spectrometry/mass spectrometry (MS/MS) sequencing. The results indicated that the digest mixtures of collagen type II and I contained lots of specific peptides and common peptides. Specific peptides could be used as index for identifying collagen type. Articular cartilage from bovine was pretreated and analyzed with the same method to determine the collagen types. The result indicated that the method developed was effective for identification of collagen types. The research provided a possible approach for collagen identification in particular tissues. (c) 2006 Elsevier B.V. All rights reserved. |
| 英文摘要 | Collagen type II and I from bovine were thermally denatured and digested with trypsin. The digest mixture was analyzed with liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS). Peptides in the digest mixture were identified by mass spectrometry/mass spectrometry (MS/MS) sequencing. The results indicated that the digest mixtures of collagen type II and I contained lots of specific peptides and common peptides. Specific peptides could be used as index for identifying collagen type. Articular cartilage from bovine was pretreated and analyzed with the same method to determine the collagen types. The result indicated that the method developed was effective for identification of collagen types. The research provided a possible approach for collagen identification in particular tissues. (c) 2006 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | IN-VIVO ; TISSUE ; ELECTROPHORESIS ; PROTEINS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000237542500015 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4009]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Univ Calif San Francisco, Sch Pharm, Dept Biopharmaceut Sci, San Francisco, CA 94143 USA |
| 推荐引用方式 GB/T 7714 | Zhang, G,Sun, A,Li, W,et al. Mass spectrometric analysis of enzymatic digestion of denatured collagen for identification of collagen type[J]. JOURNAL OF CHROMATOGRAPHY A,2006,1114(2):274-277. |
| APA | Zhang, G,Sun, A,Li, W,Liu, T,&Su, Z.(2006).Mass spectrometric analysis of enzymatic digestion of denatured collagen for identification of collagen type.JOURNAL OF CHROMATOGRAPHY A,1114(2),274-277. |
| MLA | Zhang, G,et al."Mass spectrometric analysis of enzymatic digestion of denatured collagen for identification of collagen type".JOURNAL OF CHROMATOGRAPHY A 1114.2(2006):274-277. |
入库方式： OAI收割
来源：过程工程研究所
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