One-step purification of resveratrol and polydatin from Polygonum cuspidatum (Sieb. & Zucc.) by isocratic hydrogen bond adsorption chromatography on cross-linked 12% agarose
文献类型：期刊论文
| 作者 | Gu, M.; Su, Z.-G.; Janson, J. -C. |
| 刊名 | CHROMATOGRAPHIA
 |
| 出版日期 | 2006-12-01 |
| 卷号 | 64期号:11-12页码:701-704 |
| 关键词 | column liquid chromatography hydrogen bonded adsorption chromatography resveratrol and polydatin |
| ISSN号 | 0009-5893 |
| 其他题名 | Chromatographia |
| 中文摘要 | Resveratrol and polyclatin (piceid), the major active components of the traditional Chinese medicinal herb Polygonum cuspidatum (Sieb. & Zucc.), have been separated and purified from crude root extracts in one step by isocratic hydrogen bond adsorption chromatography on cross-linked 12% agarose (Superose(TM) 12 HR 10/30). Separation was achieved by step-wise elution with mobile phases composed of mixtures of ethanol and acetic acid: 0-75 mL, 10% ethanol, 10% acetic acid; 75-150 mL, 20% ethanol, 20% acetic acid; 150-250 mL, 30% ethanol, 30% acetic acid. At a sample load of 40 mg crude extract dissolved in 0.5 mL mobile phase (corresponding to a load of 1.7 mg mL(-1) gel) a resveratrol purity of about 96% with a recovery of 61% was obtained by proper peak cutting. |
| 英文摘要 | Resveratrol and polyclatin (piceid), the major active components of the traditional Chinese medicinal herb Polygonum cuspidatum (Sieb. & Zucc.), have been separated and purified from crude root extracts in one step by isocratic hydrogen bond adsorption chromatography on cross-linked 12% agarose (Superose(TM) 12 HR 10/30). Separation was achieved by step-wise elution with mobile phases composed of mixtures of ethanol and acetic acid: 0-75 mL, 10% ethanol, 10% acetic acid; 75-150 mL, 20% ethanol, 20% acetic acid; 150-250 mL, 30% ethanol, 30% acetic acid. At a sample load of 40 mg crude extract dissolved in 0.5 mL mobile phase (corresponding to a load of 1.7 mg mL(-1) gel) a resveratrol purity of about 96% with a recovery of 61% was obtained by proper peak cutting. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | CENTRIFUGAL PARTITION CHROMATOGRAPHY ; COUNTER-CURRENT CHROMATOGRAPHY ; SEPARATION ; POLYPHENOLS ; GEL |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000243066200013 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4033]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Univ Uppsala, Uppsala Biomed Ctr, Dept Surface Biotechnol, S-75123 Uppsala, Sweden 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gu, M.,Su, Z.-G.,Janson, J. -C.. One-step purification of resveratrol and polydatin from Polygonum cuspidatum (Sieb. & Zucc.) by isocratic hydrogen bond adsorption chromatography on cross-linked 12% agarose[J]. CHROMATOGRAPHIA,2006,64(11-12):701-704. |
| APA | Gu, M.,Su, Z.-G.,&Janson, J. -C..(2006).One-step purification of resveratrol and polydatin from Polygonum cuspidatum (Sieb. & Zucc.) by isocratic hydrogen bond adsorption chromatography on cross-linked 12% agarose.CHROMATOGRAPHIA,64(11-12),701-704. |
| MLA | Gu, M.,et al."One-step purification of resveratrol and polydatin from Polygonum cuspidatum (Sieb. & Zucc.) by isocratic hydrogen bond adsorption chromatography on cross-linked 12% agarose".CHROMATOGRAPHIA 64.11-12(2006):701-704. |
入库方式： OAI收割
来源：过程工程研究所
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