Partitioning behavior of penicillin G in aqueous two phase system formed by ionic liquids and phosphate
文献类型：期刊论文
| 作者 | Liu, Qingfen; Yu, Jiang; Li, Wangliang; Hu, Xuesheng; Xia, Hansong; Liu, Huizhou; Yang, Ping |
| 刊名 | SEPARATION SCIENCE AND TECHNOLOGY
 |
| 出版日期 | 2006 |
| 卷号 | 41期号:12页码:2849-2858 |
| 关键词 | ionic liquid extraction aqueous two-phase system penicillin emulsification |
| ISSN号 | 0149-6395 |
| 其他题名 | Sep. Sci. Technol. |
| 中文摘要 | An aqueous two-phase system (ATPS) was presented with hydrophilic ionic liquid 1-butyl-3-methylimidazolium chloride ([Bmim]Cl) and NaH2PO4 aqueous solution in this paper. The partitioning behavior of penicillin G in the ATPS was investigated. Concentrations of NaH2PO4, penicillin G, and [Bmim]Cl were evaluated to determine their effects on the partition coefficient and extraction yield of penicillin G. It was found that both of partition coefficient and extraction yield strongly depended on the concentration of [Bmim]Cl, penicillin and NaH2PO4. A high extraction yield of 93% was achieved with the following parameters: NaH2PO4 center dot 2H(2)O 40% (wt%), penicillin 45000 similar to 50000 u/ml, [Bmim]Cl 20 similar to 21% (wt%). The [Bmim]Cl/NaH2PO4 system was also applied in a real filtration of penicillin G fermentation broth and the extraction yield was averaged at 91.5%. It is worthy noting that the working pH value of ATPS was at the range of 5 similar to 6, no emulsification and protein denaturation could be observed. |
| 英文摘要 | An aqueous two-phase system (ATPS) was presented with hydrophilic ionic liquid 1-butyl-3-methylimidazolium chloride ([Bmim]Cl) and NaH2PO4 aqueous solution in this paper. The partitioning behavior of penicillin G in the ATPS was investigated. Concentrations of NaH2PO4, penicillin G, and [Bmim]Cl were evaluated to determine their effects on the partition coefficient and extraction yield of penicillin G. It was found that both of partition coefficient and extraction yield strongly depended on the concentration of [Bmim]Cl, penicillin and NaH2PO4. A high extraction yield of 93% was achieved with the following parameters: NaH2PO4 center dot 2H(2)O 40% (wt%), penicillin 45000 similar to 50000 u/ml, [Bmim]Cl 20 similar to 21% (wt%). The [Bmim]Cl/NaH2PO4 system was also applied in a real filtration of penicillin G fermentation broth and the extraction yield was averaged at 91.5%. It is worthy noting that the working pH value of ATPS was at the range of 5 similar to 6, no emulsification and protein denaturation could be observed. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
| 类目[WOS] | Chemistry, Multidisciplinary ; Engineering, Chemical |
| 研究领域[WOS] | Chemistry ; Engineering |
| 关键词[WOS] | ORGANIC-SOLVENTS ; EXTRACTION ; WATER ; BROTH |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000240089800013 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4035]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Lab Separat Sci & Engn, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.N China Pharmacuet Grp, Shijiazhuang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Qingfen,Yu, Jiang,Li, Wangliang,et al. Partitioning behavior of penicillin G in aqueous two phase system formed by ionic liquids and phosphate[J]. SEPARATION SCIENCE AND TECHNOLOGY,2006,41(12):2849-2858. |
| APA | Liu, Qingfen.,Yu, Jiang.,Li, Wangliang.,Hu, Xuesheng.,Xia, Hansong.,...&Yang, Ping.(2006).Partitioning behavior of penicillin G in aqueous two phase system formed by ionic liquids and phosphate.SEPARATION SCIENCE AND TECHNOLOGY,41(12),2849-2858. |
| MLA | Liu, Qingfen,et al."Partitioning behavior of penicillin G in aqueous two phase system formed by ionic liquids and phosphate".SEPARATION SCIENCE AND TECHNOLOGY 41.12(2006):2849-2858. |
入库方式： OAI收割
来源：过程工程研究所
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