Preparation and characterization of mono-PEGylated consensus interferon by a novel polyethylene glycol derivative
文献类型：期刊论文
| 作者 | Yun, Q; Xing, WC; Mal, GG; Su, ZG |
| 刊名 | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2006-05-01 |
| 卷号 | 81期号:5页码:776-781 |
| 关键词 | consensus interferon chromatography PEGylation MALDI-TOF |
| ISSN号 | 0268-2575 |
| 其他题名 | J. Chem. Technol. Biotechnol. |
| 中文摘要 | Traditional PEGylation strategy of peptide and protein usually results in heterogeneous products with wide molecular weight distribution. Mono-PEGylated consensus interferon (C-IFN) with homogeneous molecular weight was prepared by a novel methoxypolyethylene glycol (mPEG) derivative. 2-Fluoro-1-methylpyridinium toluene-4-sulfonate (FMP) was applied to activate mPEG and a novel functionalized mPEG derivative (FMP-mPEG) was obtained. The reactivity of FMP-mPEG toward amino groups of C-IFN was evaluated, and the heterogeneity of PEGylated protein was identified by high-performance size-exclusion chromatography (HPSEC) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). Finally, the PEGylated C-IFN was separated from intact protein by cation-exchange chromatography. The results indicated that PEGylation process was dependent on pH dramatically and the modification degree was increased with the increasing molar excess of FMP-mPEG. Under optimized condition, mono-PEGylated C-IFN with molecule weight of 24.64 kDa was achieved. Further investigation of in vitro biological activities demonstrated that about 88% of antiviral activity was maintained by the mono-PEGylated C-IFN. (c) 2006 Society of Chemical Industry. |
| 英文摘要 | Traditional PEGylation strategy of peptide and protein usually results in heterogeneous products with wide molecular weight distribution. Mono-PEGylated consensus interferon (C-IFN) with homogeneous molecular weight was prepared by a novel methoxypolyethylene glycol (mPEG) derivative. 2-Fluoro-1-methylpyridinium toluene-4-sulfonate (FMP) was applied to activate mPEG and a novel functionalized mPEG derivative (FMP-mPEG) was obtained. The reactivity of FMP-mPEG toward amino groups of C-IFN was evaluated, and the heterogeneity of PEGylated protein was identified by high-performance size-exclusion chromatography (HPSEC) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). Finally, the PEGylated C-IFN was separated from intact protein by cation-exchange chromatography. The results indicated that PEGylation process was dependent on pH dramatically and the modification degree was increased with the increasing molar excess of FMP-mPEG. Under optimized condition, mono-PEGylated C-IFN with molecule weight of 24.64 kDa was achieved. Further investigation of in vitro biological activities demonstrated that about 88% of antiviral activity was maintained by the mono-PEGylated C-IFN. (c) 2006 Society of Chemical Industry. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Chemistry, Multidisciplinary ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Chemistry ; Engineering |
| 关键词[WOS] | PROTEIN PEGYLATION ; PEPTIDE ; FORM ; CHROMATOGRAPHY ; STABILITY ; RESIDUES |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000237364500008 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4044]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yun, Q,Xing, WC,Mal, GG,et al. Preparation and characterization of mono-PEGylated consensus interferon by a novel polyethylene glycol derivative[J]. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,2006,81(5):776-781. |
| APA | Yun, Q,Xing, WC,Mal, GG,&Su, ZG.(2006).Preparation and characterization of mono-PEGylated consensus interferon by a novel polyethylene glycol derivative.JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,81(5),776-781. |
| MLA | Yun, Q,et al."Preparation and characterization of mono-PEGylated consensus interferon by a novel polyethylene glycol derivative".JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 81.5(2006):776-781. |
入库方式： OAI收割
来源：过程工程研究所
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