Preparation of insulin-loaded PLA/PLGA microcapsules by a novel membrane emulsification method and its release in vitro
文献类型：期刊论文
| 作者 | Liu, Rong; Huang, Shan-Shan; Wan, Yin-Hua; Ma, Guang-Hui; Su, Zhi-Guo |
| 刊名 | COLLOIDS AND SURFACES B-BIOINTERFACES
 |
| 出版日期 | 2006-08-01 |
| 卷号 | 51期号:1页码:30-38 |
| 关键词 | SPG membrane emulsification double emulsion-solvent evaporation poly(lactide) encapsulation efficiency |
| ISSN号 | 0927-7765 |
| 其他题名 | Colloid Surf. B-Biointerfaces |
| 中文摘要 | Uniform-sized biodegradable PLA/PLGA microcapsules loading recombinant human insulin (rhI) were successfully prepared by combining a Shirasu Porous Glass (SPG) membrane emulsification technique and a double emulsion-evaporation method. An aqueous phase containing rhI was used as the inner water phase (w(1)), and PLA/PLGA and Arlacel 83 were dissolved in a mixture solvent of dichloromethane (DCM) and toluene, which was used as the oil phase (o). These two solutions were emulsified by a homogenizer to form a w(1)/o primary emulsion. The primary emulsion was permeated through the uniform pores of a SPG membrane into an outer water phase by the pressure of nitrogen gas to form the uniform w(1)/o/w(2) droplets. The solid polymer microcapsules were obtained by simply evaporating solvent from droplets. Various factors of the preparation process influencing the drug encapsulation efficiency and the drug cumulative release were investigated systemically. The results indicated that the drug encapsulation efficiency and the cumulative release were affected by the PLA/PLGA ratio, NaCl concentration in outer water phase, the inner water phase volume, rhI-loading amount, pH-value in outer water phase and the size of microcapsules. By optimizing the preparation process, the drug encapsulation efficiency was high up to 91.82%. The unique advantage of preparing drug-loaded microcapsules by membrane emulsification technique is that the size of microcapsules can be controlled accurately, and thus the drug cumulative release profile can be adjusted just by changing the size of microcapsules. Moreover, much higher encapsulation efficiency can be obtained when compared with the conventional mechanical stirring method. (c) 2006 Elsevier B.V. All rights reserved. |
| 英文摘要 | Uniform-sized biodegradable PLA/PLGA microcapsules loading recombinant human insulin (rhI) were successfully prepared by combining a Shirasu Porous Glass (SPG) membrane emulsification technique and a double emulsion-evaporation method. An aqueous phase containing rhI was used as the inner water phase (w(1)), and PLA/PLGA and Arlacel 83 were dissolved in a mixture solvent of dichloromethane (DCM) and toluene, which was used as the oil phase (o). These two solutions were emulsified by a homogenizer to form a w(1)/o primary emulsion. The primary emulsion was permeated through the uniform pores of a SPG membrane into an outer water phase by the pressure of nitrogen gas to form the uniform w(1)/o/w(2) droplets. The solid polymer microcapsules were obtained by simply evaporating solvent from droplets. Various factors of the preparation process influencing the drug encapsulation efficiency and the drug cumulative release were investigated systemically. The results indicated that the drug encapsulation efficiency and the cumulative release were affected by the PLA/PLGA ratio, NaCl concentration in outer water phase, the inner water phase volume, rhI-loading amount, pH-value in outer water phase and the size of microcapsules. By optimizing the preparation process, the drug encapsulation efficiency was high up to 91.82%. The unique advantage of preparing drug-loaded microcapsules by membrane emulsification technique is that the size of microcapsules can be controlled accurately, and thus the drug cumulative release profile can be adjusted just by changing the size of microcapsules. Moreover, much higher encapsulation efficiency can be obtained when compared with the conventional mechanical stirring method. (c) 2006 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology |
| 类目[WOS] | Biophysics ; Chemistry, Physical ; Materials Science, Biomaterials |
| 研究领域[WOS] | Biophysics ; Chemistry ; Materials Science |
| 关键词[WOS] | POLYLACTIC-ACID ; EMULSION ; MICROSPHERES ; FORMULATION ; DELIVERY ; ACETATE ; SOLVENT ; POLYMER |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000239985600005 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4049]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China 2.Beijing Univ Chem Technol, Sch Life Sci & Technol, Beijing 100029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Rong,Huang, Shan-Shan,Wan, Yin-Hua,et al. Preparation of insulin-loaded PLA/PLGA microcapsules by a novel membrane emulsification method and its release in vitro[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2006,51(1):30-38. |
| APA | Liu, Rong,Huang, Shan-Shan,Wan, Yin-Hua,Ma, Guang-Hui,&Su, Zhi-Guo.(2006).Preparation of insulin-loaded PLA/PLGA microcapsules by a novel membrane emulsification method and its release in vitro.COLLOIDS AND SURFACES B-BIOINTERFACES,51(1),30-38. |
| MLA | Liu, Rong,et al."Preparation of insulin-loaded PLA/PLGA microcapsules by a novel membrane emulsification method and its release in vitro".COLLOIDS AND SURFACES B-BIOINTERFACES 51.1(2006):30-38. |
入库方式： OAI收割
来源：过程工程研究所
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