Purification of nattokinase by reverse micelles extraction from fermentation broth: effect of temperature and phase volume ratio
文献类型：期刊论文
| 作者 | Liu, JG; Xing, JM; Chang, TS; Liu, HZ |
| 刊名 | BIOPROCESS AND BIOSYSTEMS ENGINEERING
 |
| 出版日期 | 2006-03-01 |
| 卷号 | 28期号:4页码:267-273 |
| 关键词 | reverse micelles nattokinase AOT protein purification concentration |
| ISSN号 | 1615-7591 |
| 其他题名 | Bioprocess. Biosyst. Eng. |
| 中文摘要 | Nattokinase is a novel fibrinolytic enzyme that is considered to be a promising agent for thrombosis therapy. In this study, reverse micelles extraction was applied to purify and concentrate nattokinase from fermentation broth. The effects of temperature and phase volume ratio used for the forward and backward extraction on the extraction process were examined. The optimal temperature for forward and backward extraction were 25 degrees C and 35 degrees C respectively. Nattokinase became more thermosensitive during reverse micelles extraction. And it could be enriched in the stripping phase eight times during backward extraction. It was found that nattokinase could be purified by AOT reverse micelles with up to 80% activity recovery and with a purification factor of 3.9. |
| 英文摘要 | Nattokinase is a novel fibrinolytic enzyme that is considered to be a promising agent for thrombosis therapy. In this study, reverse micelles extraction was applied to purify and concentrate nattokinase from fermentation broth. The effects of temperature and phase volume ratio used for the forward and backward extraction on the extraction process were examined. The optimal temperature for forward and backward extraction were 25 degrees C and 35 degrees C respectively. Nattokinase became more thermosensitive during reverse micelles extraction. And it could be enriched in the stripping phase eight times during backward extraction. It was found that nattokinase could be purified by AOT reverse micelles with up to 80% activity recovery and with a purification factor of 3.9. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | FIBRINOLYTIC ENZYME NATTOKINASE ; VEGETABLE CHEESE NATTO ; BACK-EXTRACTION ; PARTITIONING BEHAVIOR ; LYSOZYME EXTRACTION ; RECOVERY ; SEPARATION ; MICROEMULSIONS ; ENHANCEMENT ; ENRICHMENT |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000235753900007 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4057]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Hebei Univ Sci & Technol, Coll Biosci & Bioengn, Shijiazhuang 050018, Hebei Province, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Lab Separat Sci & Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, JG,Xing, JM,Chang, TS,et al. Purification of nattokinase by reverse micelles extraction from fermentation broth: effect of temperature and phase volume ratio[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2006,28(4):267-273. |
| APA | Liu, JG,Xing, JM,Chang, TS,&Liu, HZ.(2006).Purification of nattokinase by reverse micelles extraction from fermentation broth: effect of temperature and phase volume ratio.BIOPROCESS AND BIOSYSTEMS ENGINEERING,28(4),267-273. |
| MLA | Liu, JG,et al."Purification of nattokinase by reverse micelles extraction from fermentation broth: effect of temperature and phase volume ratio".BIOPROCESS AND BIOSYSTEMS ENGINEERING 28.4(2006):267-273. |
入库方式： OAI收割
来源：过程工程研究所
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