Rapid purification of huperzine A and B with two polystyrene based resins by preparative low-pressure liquid chromatography
文献类型：期刊论文
| 作者 | Li, XN; Ma, RY; Su, HH; Sun, HH; Ma, GH; Su, ZG; Zha, SH |
| 刊名 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
 |
| 出版日期 | 2006 |
| 卷号 | 29期号:4页码:569-582 |
| 关键词 | Huperzia serrata huperzine A huperzine B preparative low-pressure liquid chromatography purification |
| ISSN号 | 1082-6076 |
| 其他题名 | J. Liq. Chromatogr. Relat. Technol. |
| 中文摘要 | A rapid, facile, and environmentally friendly process was developed for purification of huperzine A (HupA) and huperzine B (HupB) from the Chinese traditional herb Huperzia serrata . The process consisted of two steps of preparative low-pressure liquid chromatography (LPLC) on two polystyrene based resins, successively. The first step removed a large amount of impurities and captured HupA and HupB using Amberlite XAD-4 from the herbal extraction prepared by 1% aqueous H2SO4 . This step was more efficient than multi-cycle liquid-liquid extraction as an initial separation step. The second step was able to separate HupA and HupB, employing a novel uniform polystyrene based porous microsphere named PST as the packing material. The PST column demonstrated a better separation and shorter run time compared with a commercial C-18 column. The mobile phases used in both LPLC's simply consisted of water and ethanol that are considered to be of low toxicity. Combination of the XAD-4 and PST chromatography and one step of crystallization enabled purification of HupA and HupB from 0.18% and 0.08% to 98.2% and 98.8%, respectively, with total recoveries of 82.8% and 84.3%. |
| 英文摘要 | A rapid, facile, and environmentally friendly process was developed for purification of huperzine A (HupA) and huperzine B (HupB) from the Chinese traditional herb Huperzia serrata . The process consisted of two steps of preparative low-pressure liquid chromatography (LPLC) on two polystyrene based resins, successively. The first step removed a large amount of impurities and captured HupA and HupB using Amberlite XAD-4 from the herbal extraction prepared by 1% aqueous H2SO4 . This step was more efficient than multi-cycle liquid-liquid extraction as an initial separation step. The second step was able to separate HupA and HupB, employing a novel uniform polystyrene based porous microsphere named PST as the packing material. The PST column demonstrated a better separation and shorter run time compared with a commercial C-18 column. The mobile phases used in both LPLC's simply consisted of water and ethanol that are considered to be of low toxicity. Combination of the XAD-4 and PST chromatography and one step of crystallization enabled purification of HupA and HupB from 0.18% and 0.08% to 98.2% and 98.8%, respectively, with total recoveries of 82.8% and 84.3%. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | ANTICHOLINESTERASE ACTIVITY ; RETENTION |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000235552700008 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4060]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Beijing Univ Chem Technol, Minist Educ, Key Lab Sci & Technol Controllable Chem React, Beijing 100029, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China 3.Univ Sci & Technol Beijing, Sch Civil & Environm Engn, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, XN,Ma, RY,Su, HH,et al. Rapid purification of huperzine A and B with two polystyrene based resins by preparative low-pressure liquid chromatography[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2006,29(4):569-582. |
| APA | Li, XN.,Ma, RY.,Su, HH.,Sun, HH.,Ma, GH.,...&Zha, SH.(2006).Rapid purification of huperzine A and B with two polystyrene based resins by preparative low-pressure liquid chromatography.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,29(4),569-582. |
| MLA | Li, XN,et al."Rapid purification of huperzine A and B with two polystyrene based resins by preparative low-pressure liquid chromatography".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 29.4(2006):569-582. |
入库方式： OAI收割
来源：过程工程研究所
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