Reverse micelles extraction of nattokinase: From model system to real system
文献类型：期刊论文
| 作者 | Liu, JG; Xing, JM; Chang, TS; Liu, HZ |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2006-04-01 |
| 卷号 | 51期号:7页码:796-801 |
| 关键词 | reverse micelles extraction nattokinase Subtilisin Carlsberg protein purification |
| ISSN号 | 1001-6538 |
| 其他题名 | Chin. Sci. Bull. |
| 中文摘要 | Nattokinase is a novel fibrinolytic enzyme, which is homologous to Subtilisin Carlsberg. In this paper, Subtilisin Carlsberg was taken as a model protein of nattokinase. Effects of pH, ionic strength concentration of isopropanol on the extraction of Subtilisin Carlsberg with AOT/isooctane reverse micelles system were investigated. Further, the process of reverse micelles extraction of nattokinase from fermentation broth was studied. By taking the reverse micelles of AOT/isooctane as extractant to perform a full extraction cycle, it was found that about eighty percent of the total activity of nattokinase in the fermentation broth could be recovered and the purification factor was about 2.5. Homologous protein could be reasonably used as model protein of a target protein. |
| 英文摘要 | Nattokinase is a novel fibrinolytic enzyme, which is homologous to Subtilisin Carlsberg. In this paper, Subtilisin Carlsberg was taken as a model protein of nattokinase. Effects of pH, ionic strength concentration of isopropanol on the extraction of Subtilisin Carlsberg with AOT/isooctane reverse micelles system were investigated. Further, the process of reverse micelles extraction of nattokinase from fermentation broth was studied. By taking the reverse micelles of AOT/isooctane as extractant to perform a full extraction cycle, it was found that about eighty percent of the total activity of nattokinase in the fermentation broth could be recovered and the purification factor was about 2.5. Homologous protein could be reasonably used as model protein of a target protein. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | RAT |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000237493500006 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4065]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Lab Separat Sci & Engn, Beijing 100080, Peoples R China 2.Hebei Univ Sci & Technol, Coll Biosci & Bioengn, Shijiazhuang 050018, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, JG,Xing, JM,Chang, TS,et al. Reverse micelles extraction of nattokinase: From model system to real system[J]. CHINESE SCIENCE BULLETIN,2006,51(7):796-801. |
| APA | Liu, JG,Xing, JM,Chang, TS,&Liu, HZ.(2006).Reverse micelles extraction of nattokinase: From model system to real system.CHINESE SCIENCE BULLETIN,51(7),796-801. |
| MLA | Liu, JG,et al."Reverse micelles extraction of nattokinase: From model system to real system".CHINESE SCIENCE BULLETIN 51.7(2006):796-801. |
入库方式： OAI收割
来源：过程工程研究所
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