Simultaneous determination of glycyrrhizic acid and liquiritin in Glycyrrhiza uralensis extract by HPLC with ELSD detection
文献类型：期刊论文
| 作者 | Shen, Shufeng; Chang, Zhidong; Liu, Ji; Sun, Xinghua; Hu, Xin; Liu, Huizhou |
| 刊名 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
 |
| 出版日期 | 2006 |
| 卷号 | 29期号:16页码:2387-2397 |
| 关键词 | high performance liquid chromatography (HPLC) evaporative light scattering detector (ELSD) glycyrrhizic acid liquiritin Glycyrrhiza uralensis extract |
| ISSN号 | 1082-6076 |
| 其他题名 | J. Liq. Chromatogr. Relat. Technol. |
| 中文摘要 | Simultaneous quantitative determination of glycyrrhizic acid and liquiritin in Glycyrrhiza uralensis extract was developed using a high performance liquid chromatography (HPLC) coupled to an evaporative light scattering detector (ELSD). A Luna C-18 column was used, along with a mobile phase consisting of acetonitrile and 3.0% (v/v) aqueous acetic acid in a gradient elution mode, at a flow rate of 1.0 mL/min. Parameters of ELSD were 105 degrees C for the drift tube temperature and 2.8 L/min for the gas flow-rate. Retention times of liquiritin and glycyrrhizic acid were 4.1 and 8.3 min, respectively. Logarithmic calibration curves were obtained for glycyrrhizic acid from 0.50 to 5.72 mu g (r(2)> 0.999) with a precision of 0.95% R.S.D and for liquiritin from 0.30 to 6.00 mu g (r(2)> 0.999) with a precision of 0.59% R.S.D. The limit of quantitation (LOQ) of glycyrrhizic acid and liquiritin were 168.3 ng and 200 ng (S/N=3), respectively. This method was successfully applied to quality evaluation in licorice raw materials and related commercial formulations. |
| 英文摘要 | Simultaneous quantitative determination of glycyrrhizic acid and liquiritin in Glycyrrhiza uralensis extract was developed using a high performance liquid chromatography (HPLC) coupled to an evaporative light scattering detector (ELSD). A Luna C-18 column was used, along with a mobile phase consisting of acetonitrile and 3.0% (v/v) aqueous acetic acid in a gradient elution mode, at a flow rate of 1.0 mL/min. Parameters of ELSD were 105 degrees C for the drift tube temperature and 2.8 L/min for the gas flow-rate. Retention times of liquiritin and glycyrrhizic acid were 4.1 and 8.3 min, respectively. Logarithmic calibration curves were obtained for glycyrrhizic acid from 0.50 to 5.72 mu g (r(2)> 0.999) with a precision of 0.95% R.S.D and for liquiritin from 0.30 to 6.00 mu g (r(2)> 0.999) with a precision of 0.59% R.S.D. The limit of quantitation (LOQ) of glycyrrhizic acid and liquiritin were 168.3 ng and 200 ng (S/N=3), respectively. This method was successfully applied to quality evaluation in licorice raw materials and related commercial formulations. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | CAPILLARY-ZONE-ELECTROPHORESIS ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; LICORICE ; SEPARATION ; IDENTIFICATION ; FISCH ; RADIX |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000239606200005 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4072]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Lab Separat Sci & Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shen, Shufeng,Chang, Zhidong,Liu, Ji,et al. Simultaneous determination of glycyrrhizic acid and liquiritin in Glycyrrhiza uralensis extract by HPLC with ELSD detection[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2006,29(16):2387-2397. |
| APA | Shen, Shufeng,Chang, Zhidong,Liu, Ji,Sun, Xinghua,Hu, Xin,&Liu, Huizhou.(2006).Simultaneous determination of glycyrrhizic acid and liquiritin in Glycyrrhiza uralensis extract by HPLC with ELSD detection.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,29(16),2387-2397. |
| MLA | Shen, Shufeng,et al."Simultaneous determination of glycyrrhizic acid and liquiritin in Glycyrrhiza uralensis extract by HPLC with ELSD detection".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 29.16(2006):2387-2397. |
入库方式： OAI收割
来源：过程工程研究所
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