Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering
文献类型：期刊论文
| 作者 | Luo, J; Leeman, M; Ballagi, A; Elfwing, A; Su, ZG; Janson, JC; Wahlund, KG |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2006-07-07 |
| 卷号 | 1120期号:1-2页码:158-164 |
| 关键词 | asymmetrical flow field-flow fractionation multi-angle light scattering root mean square radius hydrodynamic diameter green fluorescence protein inclusion bodies |
| ISSN号 | 0021-9673 |
| 其他题名 | J. Chromatogr. A |
| 中文摘要 | The goal of this study was to investigate the applicability of asymmetrical flow field-flow fractionation-multi-angle light scattering (AsFIFFFMALS) for size analysis of green fluorescent protein inclusion bodies (GFPIBs). The size distributions of GFPIBs prepared by various culture conditions were determined. For GFPIBs prepared at 37 degrees C the peak maximum hydrodynamic diameter (dH) first increased and then decreased with the increase of the induction times in the presence of 0.1 and 2 mM isopropyl-beta-D-thiogalactoside (IPTG). For GFPIBs prepared at 30 degrees C the peak maximum dH was constant at about 700 nm irrespectively of the induction times and IPTG concentrations. (c) 2005 Elsevier B.V. All rights reserved. |
| 英文摘要 | The goal of this study was to investigate the applicability of asymmetrical flow field-flow fractionation-multi-angle light scattering (AsFIFFFMALS) for size analysis of green fluorescent protein inclusion bodies (GFPIBs). The size distributions of GFPIBs prepared by various culture conditions were determined. For GFPIBs prepared at 37 degrees C the peak maximum hydrodynamic diameter (dH) first increased and then decreased with the increase of the induction times in the presence of 0.1 and 2 mM isopropyl-beta-D-thiogalactoside (IPTG). For GFPIBs prepared at 30 degrees C the peak maximum dH was constant at about 700 nm irrespectively of the induction times and IPTG concentrations. (c) 2005 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | ESCHERICHIA-COLI ; MAMMALIAN-CELLS ; GENE-TRANSFER ; MOLAR-MASS ; RETENTION ; DISTRIBUTIONS ; POLYSTYRENE ; EXPRESSION ; SEPARATION ; DENSITY |
| 收录类别 | ISTP ; SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000238623400020 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4074]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Lund Univ, Fac Engn, Div Tech Analyt Chem, Ctr Chem & Chem Engn, SE-22100 Lund, Sweden 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 3.Uppsala Univ, Uppsala Biomed Ctr, Dept Surface Biotechnol, SE-75123 Uppsala, Sweden |
| 推荐引用方式 GB/T 7714 | Luo, J,Leeman, M,Ballagi, A,et al. Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering[J]. JOURNAL OF CHROMATOGRAPHY A,2006,1120(1-2):158-164. |
| APA | Luo, J.,Leeman, M.,Ballagi, A.,Elfwing, A.,Su, ZG.,...&Wahlund, KG.(2006).Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering.JOURNAL OF CHROMATOGRAPHY A,1120(1-2),158-164. |
| MLA | Luo, J,et al."Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering".JOURNAL OF CHROMATOGRAPHY A 1120.1-2(2006):158-164. |
入库方式： OAI收割
来源：过程工程研究所
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