Superparamagnetic poly(methyl methacrylate) beads for nattokinase purification from fermentation broth
文献类型：期刊论文
| 作者 | Yang, Chengli; Xing, Jianmin; Guan, Yueping; Liu, Huizhou |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2006-09-01 |
| 卷号 | 72期号:3页码:616-622 |
| 关键词 | fibrinolytic enzyme nattokinase vegetable cheese natto bacillus-subtilis polymerization microspheres food |
| ISSN号 | 0175-7598 |
| 其他题名 | Appl. Microbiol. Biotechnol. |
| 中文摘要 | An effective method for purification of nattokinase from fermentation broth using magnetic poly(methyl methacrylate) (PMMA) beads immobilized with p-aminobenzamidine was proposed in this study. Firstly, magnetic PMMA beads with a narrow size distribution were prepared by spraying suspension polymerization. Then, they were highly functionalized via transesterification reaction with polyethylene glycol. The surface hydroxyl-modified magnetic beads obtained were further modified with chloroethylamine to transfer the surface amino-modified magnetic functional beads. The morphology and surface functionality of the magnetic beads were examined by scanning electron microscopy and Fourier transform infrared. An affinity ligand, p-aminobenzamidine was covalently immobilized to the amino-modified magnetic beads by the glutaraldehyde method for nattokinase purification directly from the fermentation broth. The purification factor and the recovery of the enzyme activity were found to be 8.7 and 85%, respectively. The purification of nattokinase from fermentation broth by magnetic beads only took 40 min, which shows a very fast purification of nattokinase compared to traditional purification methods. |
| 英文摘要 | An effective method for purification of nattokinase from fermentation broth using magnetic poly(methyl methacrylate) (PMMA) beads immobilized with p-aminobenzamidine was proposed in this study. Firstly, magnetic PMMA beads with a narrow size distribution were prepared by spraying suspension polymerization. Then, they were highly functionalized via transesterification reaction with polyethylene glycol. The surface hydroxyl-modified magnetic beads obtained were further modified with chloroethylamine to transfer the surface amino-modified magnetic functional beads. The morphology and surface functionality of the magnetic beads were examined by scanning electron microscopy and Fourier transform infrared. An affinity ligand, p-aminobenzamidine was covalently immobilized to the amino-modified magnetic beads by the glutaraldehyde method for nattokinase purification directly from the fermentation broth. The purification factor and the recovery of the enzyme activity were found to be 8.7 and 85%, respectively. The purification of nattokinase from fermentation broth by magnetic beads only took 40 min, which shows a very fast purification of nattokinase compared to traditional purification methods. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | FIBRINOLYTIC ENZYME NATTOKINASE ; VEGETABLE CHEESE NATTO ; BACILLUS-SUBTILIS ; POLYMERIZATION ; MICROSPHERES ; FOOD |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000239956200027 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4088]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Lab Separat Sci & Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Chengli,Xing, Jianmin,Guan, Yueping,et al. Superparamagnetic poly(methyl methacrylate) beads for nattokinase purification from fermentation broth[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2006,72(3):616-622. |
| APA | Yang, Chengli,Xing, Jianmin,Guan, Yueping,&Liu, Huizhou.(2006).Superparamagnetic poly(methyl methacrylate) beads for nattokinase purification from fermentation broth.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,72(3),616-622. |
| MLA | Yang, Chengli,et al."Superparamagnetic poly(methyl methacrylate) beads for nattokinase purification from fermentation broth".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 72.3(2006):616-622. |
入库方式： OAI收割
来源：过程工程研究所
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