Superparamagnetic silica nanoparticles with immobilized metal affinity ligands for protein adsorption
文献类型：期刊论文
| 作者 | Ma, ZY; Guan, YP; Liu, HZ |
| 刊名 | JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
 |
| 出版日期 | 2006-06-01 |
| 卷号 | 301期号:2页码:469-477 |
| 关键词 | superparamagnetic silica nanoparticles immobilized metal affinity separation protein adsorption |
| ISSN号 | 0304-8853 |
| 其他题名 | J. Magn. Magn. Mater. |
| 中文摘要 | Superparamagnetic silica-coated magnetite (Fe3O4) nanoparticles with immobilized metal affinity ligands were prepared for protein adsorption. First, magnetite nanoparticles were synthesized by co-precipitating Fe2+ and Fe3+ in an ammonia solution. Then silica was coated on the Fe3O4 nanoparticles using a sol-gel method to obtain magnetic silica nanoparticles. The condensation product of 3-Glycidoxypropyltrimethoxysilane (GLYMO) and iminodiacetic acid (IDA) was immobilized on them and after charged with Cu2+. the magnetic silica nanoparticles with immobilized Cu2+ were applied for the adsorption of bovine serum albumin (BSA). Scanning electron micrograph showed that the magnetic silica nanoparticles with an average size of 190 nm were well dispersed without aggregation. X-ray diffraction showed the spinel structure for the magnetite particles coated with silica. Magnetic measurement revealed the magnetic silica nanoparticles were superparamagnetic and the saturation magnetization was about 15.0emu/g. Protein adsorption results showed that the nanoparticles had high adsorption capacity for BSA (73 mg/g) and low nonspecific adsorption. The regeneration of these nanoparticles was also studied. (c) 2005 Elsevier B.V. All rights reserved. |
| 英文摘要 | Superparamagnetic silica-coated magnetite (Fe3O4) nanoparticles with immobilized metal affinity ligands were prepared for protein adsorption. First, magnetite nanoparticles were synthesized by co-precipitating Fe2+ and Fe3+ in an ammonia solution. Then silica was coated on the Fe3O4 nanoparticles using a sol-gel method to obtain magnetic silica nanoparticles. The condensation product of 3-Glycidoxypropyltrimethoxysilane (GLYMO) and iminodiacetic acid (IDA) was immobilized on them and after charged with Cu2+. the magnetic silica nanoparticles with immobilized Cu2+ were applied for the adsorption of bovine serum albumin (BSA). Scanning electron micrograph showed that the magnetic silica nanoparticles with an average size of 190 nm were well dispersed without aggregation. X-ray diffraction showed the spinel structure for the magnetite particles coated with silica. Magnetic measurement revealed the magnetic silica nanoparticles were superparamagnetic and the saturation magnetization was about 15.0emu/g. Protein adsorption results showed that the nanoparticles had high adsorption capacity for BSA (73 mg/g) and low nonspecific adsorption. The regeneration of these nanoparticles was also studied. (c) 2005 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Technology ; Physical Sciences |
| 类目[WOS] | Materials Science, Multidisciplinary ; Physics, Condensed Matter |
| 研究领域[WOS] | Materials Science ; Physics |
| 关键词[WOS] | IRON-OXIDE NANOPARTICLES ; PARTICLES ; CHROMATOGRAPHY ; PURIFICATION ; MEMBRANES ; MAGNETITE ; SUPPORTS ; FE3O4 |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000236683100027 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4089]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Lab Separat Sci & Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, ZY,Guan, YP,Liu, HZ. Superparamagnetic silica nanoparticles with immobilized metal affinity ligands for protein adsorption[J]. JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS,2006,301(2):469-477. |
| APA | Ma, ZY,Guan, YP,&Liu, HZ.(2006).Superparamagnetic silica nanoparticles with immobilized metal affinity ligands for protein adsorption.JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS,301(2),469-477. |
| MLA | Ma, ZY,et al."Superparamagnetic silica nanoparticles with immobilized metal affinity ligands for protein adsorption".JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS 301.2(2006):469-477. |
入库方式： OAI收割
来源：过程工程研究所
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