Synthesis of magnetic silica nanospheres with metal ligands and application in affinity separation of proteins
文献类型：期刊论文
| 作者 | Ma, ZY; Liu, XQ; Guan, YP; Liu, HZ |
| 刊名 | COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
 |
| 出版日期 | 2006-03-01 |
| 卷号 | 275期号:1-3页码:87-91 |
| 关键词 | magnetic silica nanospheres metal affinity ligands iminodiacetic acid protein separation |
| ISSN号 | 0927-7757 |
| 其他题名 | Colloid Surf. A-Physicochem. Eng. Asp. |
| 中文摘要 | Magnetic silica nanospheres with immobilized metal ligands were prepared for the separation of proteins. First, silica-coated magnetite nanospheres were prepared by the deposition of silica onto ultrafine (about 8 mu) magnetite particles via controlled hydrolysis of sodium silicate. Then through reaction of iminodiacetic acid (IDA) with 3-alycidoxypropyltrilliethoxysilane (GLYMO) and immobilization of them onto the above magnetic silica nanospheres, novel magnetic affinity adsorbents with IDA chelating groups were obtained. Charged with Zn2+, the magnetic nanospheres were applied to separate a model protein mixture of bovine hemoglobin (BHb) and bovine serum albumin (BSA). They could be separated completely and showed low non-specific adsorption. The morphology and magnetic properties of magnetic silica nanospheres were investigated by TEM and VSM. The nanospheres have an average size of 200 mu and possess superparamagnetic characteristics. These magnetic silica nanospheres with metal ligands can be used efficiently in protein affinity separation and purification. (c) 2005 Elsevier B.V. All rights reserved. |
| 英文摘要 | Magnetic silica nanospheres with immobilized metal ligands were prepared for the separation of proteins. First, silica-coated magnetite nanospheres were prepared by the deposition of silica onto ultrafine (about 8 mu) magnetite particles via controlled hydrolysis of sodium silicate. Then through reaction of iminodiacetic acid (IDA) with 3-alycidoxypropyltrilliethoxysilane (GLYMO) and immobilization of them onto the above magnetic silica nanospheres, novel magnetic affinity adsorbents with IDA chelating groups were obtained. Charged with Zn2+, the magnetic nanospheres were applied to separate a model protein mixture of bovine hemoglobin (BHb) and bovine serum albumin (BSA). They could be separated completely and showed low non-specific adsorption. The morphology and magnetic properties of magnetic silica nanospheres were investigated by TEM and VSM. The nanospheres have an average size of 200 mu and possess superparamagnetic characteristics. These magnetic silica nanospheres with metal ligands can be used efficiently in protein affinity separation and purification. (c) 2005 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Physical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | IRON-OXIDE NANOPARTICLES ; PARTICLES ; IMMOBILIZATION ; MICROEMULSION ; PURIFICATION ; SUPPORT ; BINDING ; GEL ; PH |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000235523100014 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4098]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Lab Separat Sci & Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, ZY,Liu, XQ,Guan, YP,et al. Synthesis of magnetic silica nanospheres with metal ligands and application in affinity separation of proteins[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2006,275(1-3):87-91. |
| APA | Ma, ZY,Liu, XQ,Guan, YP,&Liu, HZ.(2006).Synthesis of magnetic silica nanospheres with metal ligands and application in affinity separation of proteins.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,275(1-3),87-91. |
| MLA | Ma, ZY,et al."Synthesis of magnetic silica nanospheres with metal ligands and application in affinity separation of proteins".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 275.1-3(2006):87-91. |
入库方式： OAI收割
来源：过程工程研究所
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