2004 SIVB Congress Symposium Proceedings "Thinking outside the cell": Optimized chemodiversity in protoplast-derived lines of St. John's wort (Hypericum perforatum L.)
文献类型：期刊论文
| 作者 | Pan, ZG; Liu, CZ; Murch, SJ; Saxena, PK |
| 刊名 | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
 |
| 出版日期 | 2005-05-01 |
| 卷号 | 41期号:3页码:226-231 |
| 关键词 | antioxidant activity Hypericum perforatum L. phytochemicals plant regeneration protoplasts |
| ISSN号 | 1054-5476 |
| 其他题名 | In Vitro Cell. Dev. Biol.-Plant |
| 中文摘要 | A procedure for protoplast isolation and plant regeneration of St. John's wort has been developed to utilize cell-to-cell variability for optimum production of valuable medicinal compounds. Calluses, induced from hypocotyl segments of St. John's wort seedlings, were used for protoplast isolation, induction of sustained cell division, and ultimately, plant regeneration. Callus-isolated protoplasts at a density of 2.0x10(5) per ml were embedded in 0.6% Na-alginate blocks and cultured in a medium containing modified Murashige and Skoog (MS) salts, 2.5 mu M 6-benzylaminopurine (BA), 5.0 mu M alpha-naphthaleneacetic acid (NAA), and 0.5 mol l(-1) glucose. Protoplast-derived colonies formed compact calluses when transferred onto 0.35% gellan gum-solidified MS medium supplemented with 2.5 mu M BA and 2.5 mu M NAA. Shoot organogenesis from the protoplast-derived callus was induced on MS medium supplemented with 5 mu M thidiazuron. Complete plantlets were obtained from the regenerated shoots on MS basal medium. A greater than 3-fold variation of antioxidant activity was observed among the protoplast-derived plantlets and chemically distinct germplasm lines were selected on the basis of phytochemical profiles. The protoplast to plant regeneration protocol developed in this study provides the foundation for development of novel genotypes with potential expansion of the genetic diversity through somatic hybridization, and organelle transplantation. |
| 英文摘要 | A procedure for protoplast isolation and plant regeneration of St. John's wort has been developed to utilize cell-to-cell variability for optimum production of valuable medicinal compounds. Calluses, induced from hypocotyl segments of St. John's wort seedlings, were used for protoplast isolation, induction of sustained cell division, and ultimately, plant regeneration. Callus-isolated protoplasts at a density of 2.0x10(5) per ml were embedded in 0.6% Na-alginate blocks and cultured in a medium containing modified Murashige and Skoog (MS) salts, 2.5 mu M 6-benzylaminopurine (BA), 5.0 mu M alpha-naphthaleneacetic acid (NAA), and 0.5 mol l(-1) glucose. Protoplast-derived colonies formed compact calluses when transferred onto 0.35% gellan gum-solidified MS medium supplemented with 2.5 mu M BA and 2.5 mu M NAA. Shoot organogenesis from the protoplast-derived callus was induced on MS medium supplemented with 5 mu M thidiazuron. Complete plantlets were obtained from the regenerated shoots on MS basal medium. A greater than 3-fold variation of antioxidant activity was observed among the protoplast-derived plantlets and chemically distinct germplasm lines were selected on the basis of phytochemical profiles. The protoplast to plant regeneration protocol developed in this study provides the foundation for development of novel genotypes with potential expansion of the genetic diversity through somatic hybridization, and organelle transplantation. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Plant Sciences ; Cell Biology ; Developmental Biology |
| 研究领域[WOS] | Plant Sciences ; Cell Biology ; Developmental Biology |
| 关键词[WOS] | PLANT-REGENERATION ; MESOPHYLL PROTOPLASTS ; HYPERFORIN ; CULTURES ; THIDIAZURON ; INHIBITION |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000230506000006 |
| 公开日期 | 2013-10-25 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4129]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pan, ZG,Liu, CZ,Murch, SJ,et al. 2004 SIVB Congress Symposium Proceedings "Thinking outside the cell": Optimized chemodiversity in protoplast-derived lines of St. John's wort (Hypericum perforatum L.)[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,2005,41(3):226-231. |
| APA | Pan, ZG,Liu, CZ,Murch, SJ,&Saxena, PK.(2005).2004 SIVB Congress Symposium Proceedings "Thinking outside the cell": Optimized chemodiversity in protoplast-derived lines of St. John's wort (Hypericum perforatum L.).IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,41(3),226-231. |
| MLA | Pan, ZG,et al."2004 SIVB Congress Symposium Proceedings "Thinking outside the cell": Optimized chemodiversity in protoplast-derived lines of St. John's wort (Hypericum perforatum L.)".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 41.3(2005):226-231. |
入库方式： OAI收割
来源：过程工程研究所
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