Covalent immobilization of albumin on micron-sized magnetic poly(methyl methacrylate-divinylbenzene-glycidyl methacrylate) microspheres prepared by modified suspension polymerization
文献类型：期刊论文
| 作者 | Ma, ZY; Guan, YP; Liu, XQ; Liu, HZ |
| 刊名 | POLYMERS FOR ADVANCED TECHNOLOGIES
 |
| 出版日期 | 2005-07-01 |
| 卷号 | 16期号:7页码:554-558 |
| 关键词 | magnetic microspheres covalent immobilization functionalization of polymers proteins modification |
| ISSN号 | 1042-7147 |
| 其他题名 | Polym. Adv. Technol. |
| 中文摘要 | Micron-sized magnetic poly(methyl methacrylate-divinylbenzene-glycidyI methacrylate) microspheres were prepared by a modified suspension polymerization in the presence of oleic acid-coated magnetite nanoparticles. The magnetic microspheres were functionalized by reacting the epoxy groups with ammonia solution to provide amino groups. After activated with glutaraldehyde (GA), bovine serum albumin was covalently immobilized on these magnetic microspheres. The influence of initial protein concentration, pH and ionic strength of the protein solution on covalent immobilization was studied. Scanning electron micrographs showed that the magnetic microspheres had an average size of 6.4 mu m and relative narrow size distribution. Magnetic measurement revealed the magnetic microspheres were superparamagetic with saturation magnetization of 7.32 emu./g. The successful amination of the magnetic microspheres was confirmed by Fourier transform infrared spectroscopy (FT-IR). Copyright (c) 2005 John Wiley & Sons, Ltd. |
| 英文摘要 | Micron-sized magnetic poly(methyl methacrylate-divinylbenzene-glycidyI methacrylate) microspheres were prepared by a modified suspension polymerization in the presence of oleic acid-coated magnetite nanoparticles. The magnetic microspheres were functionalized by reacting the epoxy groups with ammonia solution to provide amino groups. After activated with glutaraldehyde (GA), bovine serum albumin was covalently immobilized on these magnetic microspheres. The influence of initial protein concentration, pH and ionic strength of the protein solution on covalent immobilization was studied. Scanning electron micrographs showed that the magnetic microspheres had an average size of 6.4 mu m and relative narrow size distribution. Magnetic measurement revealed the magnetic microspheres were superparamagetic with saturation magnetization of 7.32 emu./g. The successful amination of the magnetic microspheres was confirmed by Fourier transform infrared spectroscopy (FT-IR). Copyright (c) 2005 John Wiley & Sons, Ltd. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Polymer Science |
| 研究领域[WOS] | Polymer Science |
| 关键词[WOS] | ADSORPTION ; PARTICLES ; PROTEIN ; POLYSTYRENE ; ANTIBODIES ; SEPARATION ; STYRENE ; SURFACE ; ENZYME ; LATEX |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000230495400008 |
| 公开日期 | 2013-10-25 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4139]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, Lab Separat Sci & Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, ZY,Guan, YP,Liu, XQ,et al. Covalent immobilization of albumin on micron-sized magnetic poly(methyl methacrylate-divinylbenzene-glycidyl methacrylate) microspheres prepared by modified suspension polymerization[J]. POLYMERS FOR ADVANCED TECHNOLOGIES,2005,16(7):554-558. |
| APA | Ma, ZY,Guan, YP,Liu, XQ,&Liu, HZ.(2005).Covalent immobilization of albumin on micron-sized magnetic poly(methyl methacrylate-divinylbenzene-glycidyl methacrylate) microspheres prepared by modified suspension polymerization.POLYMERS FOR ADVANCED TECHNOLOGIES,16(7),554-558. |
| MLA | Ma, ZY,et al."Covalent immobilization of albumin on micron-sized magnetic poly(methyl methacrylate-divinylbenzene-glycidyl methacrylate) microspheres prepared by modified suspension polymerization".POLYMERS FOR ADVANCED TECHNOLOGIES 16.7(2005):554-558. |
入库方式： OAI收割
来源：过程工程研究所
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