Disuccinimidyl suberate cross-linked hemoglobin as a novel red blood cell substitute
文献类型：期刊论文
| 作者 | Lu, XL; Zheng, CY; Xu, YH; Su, ZG |
| 刊名 | SCIENCE IN CHINA SERIES C-LIFE SCIENCES
 |
| 出版日期 | 2005-02-01 |
| 卷号 | 48期号:1页码:49-60 |
| 关键词 | hemoglobin disuccinimidyl suberate cross-link red blood cell substitute |
| ISSN号 | 1006-9305 |
| 其他题名 | Sci. China Ser. C-Life Sci. |
| 中文摘要 | Disuccinimidyl suberate (DSS) intramolecularly cross-linked hemoglobin (Hb) was developed as a novel red blood cell substitute. A multi-angle laser light scattering detector coupled with size exclusion HPLC was applied to determine the molecular weight of the modified Hb. SIDS-PAGE was also used as a complement. It was proved that 83.8% of the product was intramolecularly cross-linked Hb with weight-average molecular weights (Mw) of 67.5 kD, 12% was dimeric Hb with Mw of 146.6 kD, and 4.2% was trimeric Hb with Mw of 306.4 kD. The tetramer structure of the cross-linked Hb was stable as shown in size-exclusion chromatography using a mobile phase containing 1 mol/L MgCl2. Analysis by LC-MS demonstrated that the reaction of DSS with Hb mainly took place between the two alpha subunits within a Hb molecule, resulting in stabilization of the tetramer structure. However, the cross-linking was not site-specific. The P-50 Of the cross-linked Hb decreased from 21.8 mmHg to 14.3 mmHg, and the Hill coefficient decreased from 2.22 to 1.41. Result of isoelectric focusing showed that the pl of DSS cross-linked Hb was in the range of 4.6-5.2, similar to that of serum albumin. The safety of DSS cross-linked Hb was favored by animal tests on rats and guinea pigs. Exchange transfusion experiment with DSS cross-linked Hb using rats as a model indicated no pressor effect or other significant side effects. The characteristics and properties of DSS cross-linked Hb were also compared with that of diaspirin cross-linked Hb reported in the literature. |
| 英文摘要 | Disuccinimidyl suberate (DSS) intramolecularly cross-linked hemoglobin (Hb) was developed as a novel red blood cell substitute. A multi-angle laser light scattering detector coupled with size exclusion HPLC was applied to determine the molecular weight of the modified Hb. SIDS-PAGE was also used as a complement. It was proved that 83.8% of the product was intramolecularly cross-linked Hb with weight-average molecular weights (Mw) of 67.5 kD, 12% was dimeric Hb with Mw of 146.6 kD, and 4.2% was trimeric Hb with Mw of 306.4 kD. The tetramer structure of the cross-linked Hb was stable as shown in size-exclusion chromatography using a mobile phase containing 1 mol/L MgCl2. Analysis by LC-MS demonstrated that the reaction of DSS with Hb mainly took place between the two alpha subunits within a Hb molecule, resulting in stabilization of the tetramer structure. However, the cross-linking was not site-specific. The P-50 Of the cross-linked Hb decreased from 21.8 mmHg to 14.3 mmHg, and the Hill coefficient decreased from 2.22 to 1.41. Result of isoelectric focusing showed that the pl of DSS cross-linked Hb was in the range of 4.6-5.2, similar to that of serum albumin. The safety of DSS cross-linked Hb was favored by animal tests on rats and guinea pigs. Exchange transfusion experiment with DSS cross-linked Hb using rats as a model indicated no pressor effect or other significant side effects. The characteristics and properties of DSS cross-linked Hb were also compared with that of diaspirin cross-linked Hb reported in the literature. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biology |
| 研究领域[WOS] | Life Sciences & Biomedicine - Other Topics |
| 关键词[WOS] | POLYETHYLENE-GLYCOL ; PROTEIN ; PURIFICATION ; PHARMACOLOGY ; DERIVATIVES ; RECEPTOR ; SAFETY |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000228190700007 |
| 公开日期 | 2013-10-25 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4149]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Beijing Univ Chem Technol, Sch Life Sci & Technol, Beijing 100029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, XL,Zheng, CY,Xu, YH,et al. Disuccinimidyl suberate cross-linked hemoglobin as a novel red blood cell substitute[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2005,48(1):49-60. |
| APA | Lu, XL,Zheng, CY,Xu, YH,&Su, ZG.(2005).Disuccinimidyl suberate cross-linked hemoglobin as a novel red blood cell substitute.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,48(1),49-60. |
| MLA | Lu, XL,et al."Disuccinimidyl suberate cross-linked hemoglobin as a novel red blood cell substitute".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 48.1(2005):49-60. |
入库方式： OAI收割
来源：过程工程研究所
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