中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Effect of sucrose on nisin production in batch and fed-batch culture by Lactococcus lactis

文献类型:期刊论文

作者Lv, WH; Cong, W; Cai, ZL
刊名JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
出版日期2005-05-01
卷号80期号:5页码:511-514
关键词nisin pH feedback fed-batch culture sucrose
ISSN号0268-2575
其他题名J. Chem. Technol. Biotechnol.
中文摘要The effects of sucrose on cell growth and nisin production by Lactococcus lactis were investigated in batch and pH feed-back controlled fed-batch cultures. In batch cultures, nisin titer reached its maximum, 2658 IU cm(-3), at the initial sucrose concentration of 30 g dm(-3). With sucrose concentrations higher than 30 g dm(-3), nisin production decreased while the biomass was not influenced significantly. By using the pH feed-back controlled method, residual sucrose concentration could be controlled well in fed-batch cultures and three conditions (sucrose maintained at 2, 16, 20 g dm(-3), respectively) were evaluated. Maintaining a low sucrose concentration at 2 g dm(-3) during feeding favored nisin biosynthesis, and the maximum nisin titer obtained was 4961 IU cm(-3) compared with 3370 IUcm(-3) (16g sucrose dm-3)and 3498 IU cm(-3) (20 g sucrose dm-3), respectively. (c) 2005 Society of Chemical Industry
英文摘要The effects of sucrose on cell growth and nisin production by Lactococcus lactis were investigated in batch and pH feed-back controlled fed-batch cultures. In batch cultures, nisin titer reached its maximum, 2658 IU cm(-3), at the initial sucrose concentration of 30 g dm(-3). With sucrose concentrations higher than 30 g dm(-3), nisin production decreased while the biomass was not influenced significantly. By using the pH feed-back controlled method, residual sucrose concentration could be controlled well in fed-batch cultures and three conditions (sucrose maintained at 2, 16, 20 g dm(-3), respectively) were evaluated. Maintaining a low sucrose concentration at 2 g dm(-3) during feeding favored nisin biosynthesis, and the maximum nisin titer obtained was 4961 IU cm(-3) compared with 3370 IUcm(-3) (16g sucrose dm-3)and 3498 IU cm(-3) (20 g sucrose dm-3), respectively. (c) 2005 Society of Chemical Industry
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology
类目[WOS]Biotechnology & Applied Microbiology ; Chemistry, Multidisciplinary ; Engineering, Chemical
研究领域[WOS]Biotechnology & Applied Microbiology ; Chemistry ; Engineering
关键词[WOS]FERMENTATIONS ; GROWTH
收录类别SCI
原文出处://WOS:000228562200004
语种英语
WOS记录号WOS:000228562200004
公开日期2013-10-25
版本出版稿
源URL[http://ir.ipe.ac.cn/handle/122111/4152]  
专题过程工程研究所_研究所(批量导入)
作者单位Chinese Acad Sci, Grad Sch, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
推荐引用方式
GB/T 7714
Lv, WH,Cong, W,Cai, ZL. Effect of sucrose on nisin production in batch and fed-batch culture by Lactococcus lactis[J]. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,2005,80(5):511-514.
APA Lv, WH,Cong, W,&Cai, ZL.(2005).Effect of sucrose on nisin production in batch and fed-batch culture by Lactococcus lactis.JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,80(5),511-514.
MLA Lv, WH,et al."Effect of sucrose on nisin production in batch and fed-batch culture by Lactococcus lactis".JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 80.5(2005):511-514.

入库方式: OAI收割

来源:过程工程研究所

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