Effect of sucrose on nisin production in batch and fed-batch culture by Lactococcus lactis
文献类型：期刊论文
| 作者 | Lv, WH; Cong, W; Cai, ZL |
| 刊名 | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2005-05-01 |
| 卷号 | 80期号:5页码:511-514 |
| 关键词 | nisin pH feedback fed-batch culture sucrose |
| ISSN号 | 0268-2575 |
| 其他题名 | J. Chem. Technol. Biotechnol. |
| 中文摘要 | The effects of sucrose on cell growth and nisin production by Lactococcus lactis were investigated in batch and pH feed-back controlled fed-batch cultures. In batch cultures, nisin titer reached its maximum, 2658 IU cm(-3), at the initial sucrose concentration of 30 g dm(-3). With sucrose concentrations higher than 30 g dm(-3), nisin production decreased while the biomass was not influenced significantly. By using the pH feed-back controlled method, residual sucrose concentration could be controlled well in fed-batch cultures and three conditions (sucrose maintained at 2, 16, 20 g dm(-3), respectively) were evaluated. Maintaining a low sucrose concentration at 2 g dm(-3) during feeding favored nisin biosynthesis, and the maximum nisin titer obtained was 4961 IU cm(-3) compared with 3370 IUcm(-3) (16g sucrose dm-3)and 3498 IU cm(-3) (20 g sucrose dm-3), respectively. (c) 2005 Society of Chemical Industry |
| 英文摘要 | The effects of sucrose on cell growth and nisin production by Lactococcus lactis were investigated in batch and pH feed-back controlled fed-batch cultures. In batch cultures, nisin titer reached its maximum, 2658 IU cm(-3), at the initial sucrose concentration of 30 g dm(-3). With sucrose concentrations higher than 30 g dm(-3), nisin production decreased while the biomass was not influenced significantly. By using the pH feed-back controlled method, residual sucrose concentration could be controlled well in fed-batch cultures and three conditions (sucrose maintained at 2, 16, 20 g dm(-3), respectively) were evaluated. Maintaining a low sucrose concentration at 2 g dm(-3) during feeding favored nisin biosynthesis, and the maximum nisin titer obtained was 4961 IU cm(-3) compared with 3370 IUcm(-3) (16g sucrose dm-3)and 3498 IU cm(-3) (20 g sucrose dm-3), respectively. (c) 2005 Society of Chemical Industry |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Chemistry, Multidisciplinary ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Chemistry ; Engineering |
| 关键词[WOS] | FERMENTATIONS ; GROWTH |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000228562200004 |
| 公开日期 | 2013-10-25 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4152]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Grad Sch, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lv, WH,Cong, W,Cai, ZL. Effect of sucrose on nisin production in batch and fed-batch culture by Lactococcus lactis[J]. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,2005,80(5):511-514. |
| APA | Lv, WH,Cong, W,&Cai, ZL.(2005).Effect of sucrose on nisin production in batch and fed-batch culture by Lactococcus lactis.JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,80(5),511-514. |
| MLA | Lv, WH,et al."Effect of sucrose on nisin production in batch and fed-batch culture by Lactococcus lactis".JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 80.5(2005):511-514. |
入库方式： OAI收割
来源：过程工程研究所
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