Effects of co-expression of two higher plants genes ALD and TPI in Anabaena sp PCC7120 on photosynthetic CO2 fixation
文献类型：期刊论文
| 作者 | Kang, RJ; Shi, DJ; Cong, W; Ma, WM; Cai, ZL; Fan, OY |
| 刊名 | ENZYME AND MICROBIAL TECHNOLOGY
 |
| 出版日期 | 2005-03-02 |
| 卷号 | 36期号:4页码:600-604 |
| ISSN号 | 0141-0229 |
| 关键词 | transgenic cyanobacteria Anabaena sp PCC7120 fructose 1 triosephosphate isomerase (TPI) Calvin cycle carbonic anhydrases (CA) airlift photobioreactor 6-bisphosphate aldolase (ALD) |
| 其他题名 | Enzyme Microb. Technol. |
| 中文摘要 | For the regulation of photosynthesis at gene level, the genes encoding fructose-1,6-bisphosphate aldolase (ALD) and triosephosphate isomerase (TPI) were transferred into filamentous, heterocystour cyanobacterium Anabaena sp. PCC7120, which were from spinach and rice, respectively. Cells were grown in the medium supplemented with CO2. The growth parameters, photosynthesis, enzyme activity of ALD and TPI and carbonic anhydrases activities were measured and compared between transgenic and wild type cells. Transgenic cells had higher ALD and TPI enzyme activities than the wild type cells at all experiment conditions. Both cell growth and photosynthesis activities were improved under elevated CO2, especially for transgenic cells. Total intracellular CA activities were decreased with the increasing of NaHCO3 concentrations, and increased with the increasing of CO2 concentrations. Transgenic cells showed comparative advantages over the wild type cells in all experiments. These data indicated that the overexpress of foreign ALD and TPI affected the photosynthetic carbon fixation process through the accelerating of carbon flux. (C) 2004 Elsevier Inc. All rights reserved. |
| 英文摘要 | For the regulation of photosynthesis at gene level, the genes encoding fructose-1,6-bisphosphate aldolase (ALD) and triosephosphate isomerase (TPI) were transferred into filamentous, heterocystour cyanobacterium Anabaena sp. PCC7120, which were from spinach and rice, respectively. Cells were grown in the medium supplemented with CO2. The growth parameters, photosynthesis, enzyme activity of ALD and TPI and carbonic anhydrases activities were measured and compared between transgenic and wild type cells. Transgenic cells had higher ALD and TPI enzyme activities than the wild type cells at all experiment conditions. Both cell growth and photosynthesis activities were improved under elevated CO2, especially for transgenic cells. Total intracellular CA activities were decreased with the increasing of NaHCO3 concentrations, and increased with the increasing of CO2 concentrations. Transgenic cells showed comparative advantages over the wild type cells in all experiments. These data indicated that the overexpress of foreign ALD and TPI affected the photosynthetic carbon fixation process through the accelerating of carbon flux. (C) 2004 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | CYANOBACTERIA ; MECHANISMS ; ALDOLASE ; CARBON |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000227027000032 |
| 公开日期 | 2013-10-25 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4154]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300450, Peoples R China |
| 推荐引用方式 GB/T 7714 | Kang, RJ,Shi, DJ,Cong, W,et al. Effects of co-expression of two higher plants genes ALD and TPI in Anabaena sp PCC7120 on photosynthetic CO2 fixation[J]. ENZYME AND MICROBIAL TECHNOLOGY,2005,36(4):600-604. |
| APA | Kang, RJ,Shi, DJ,Cong, W,Ma, WM,Cai, ZL,&Fan, OY.(2005).Effects of co-expression of two higher plants genes ALD and TPI in Anabaena sp PCC7120 on photosynthetic CO2 fixation.ENZYME AND MICROBIAL TECHNOLOGY,36(4),600-604. |
| MLA | Kang, RJ,et al."Effects of co-expression of two higher plants genes ALD and TPI in Anabaena sp PCC7120 on photosynthetic CO2 fixation".ENZYME AND MICROBIAL TECHNOLOGY 36.4(2005):600-604. |
入库方式： OAI收割
来源：过程工程研究所
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