Expression of a wheat cytochrome P450 monooxygenase in yeast and its inhibition by glyphosate
文献类型：期刊论文
| 作者 | Xiang, WS; Wang, XJ; Ren, TR; Ju, XL |
| 刊名 | PEST MANAGEMENT SCIENCE
 |
| 出版日期 | 2005-04-01 |
| 卷号 | 61期号:4页码:402-406 |
| 关键词 | wheat cytochrome P450 heterologous expression inhibition glyphosate chlorsulfuron |
| ISSN号 | 1526-498X |
| 其他题名 | Pest Manag. Sci. |
| 中文摘要 | Glyphosate is a non-selective herbicide which acts by inhibiting 5-enolpyruvylshikimate-3-phosphate synthase. Wheat cytochrome P450 monooxygenase specifically catalyzes the metabolism of some sulfonylurea herbicides such as chlorsulfuron and triasulfuron. Here we report that glyphosate is an inhibitor of a wheat cytochrome (CYP71C6v1), the cDNA of which was amplified by RT-PCR and heterologously expressed in yeast. The microsomal fractions derived from this strain had a Soret peak at 502 nm in the reduced carbon monoxide difference spectrum, which is a typical spectral characteristic. The addition of glyphosate to the microsomal protein resulted in a Type II spectrum indicative of binding via the nitrogen group to haem of cytochrome P450 as a sixth ligand. A spectral dissociation constant, K-s of 70 mu mol litre(-1) was observed and an IC50 of 11 mu mol litre(-1) was found for glyphosate inhibition of CYP7lC6vl P450 activity. (c) 2004 Society of Chemical Industry. |
| 英文摘要 | Glyphosate is a non-selective herbicide which acts by inhibiting 5-enolpyruvylshikimate-3-phosphate synthase. Wheat cytochrome P450 monooxygenase specifically catalyzes the metabolism of some sulfonylurea herbicides such as chlorsulfuron and triasulfuron. Here we report that glyphosate is an inhibitor of a wheat cytochrome (CYP71C6v1), the cDNA of which was amplified by RT-PCR and heterologously expressed in yeast. The microsomal fractions derived from this strain had a Soret peak at 502 nm in the reduced carbon monoxide difference spectrum, which is a typical spectral characteristic. The addition of glyphosate to the microsomal protein resulted in a Type II spectrum indicative of binding via the nitrogen group to haem of cytochrome P450 as a sixth ligand. A spectral dissociation constant, K-s of 70 mu mol litre(-1) was observed and an IC50 of 11 mu mol litre(-1) was found for glyphosate inhibition of CYP7lC6vl P450 activity. (c) 2004 Society of Chemical Industry. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Agronomy ; Entomology |
| 研究领域[WOS] | Agriculture ; Entomology |
| 关键词[WOS] | PLANT CYTOCHROME-P450 ; FUNCTIONAL EXPRESSION ; MOLECULAR-CLONING ; METABOLISM ; HYDROXYLATION ; MICROSOMES ; CYP76B1 ; MAIZE ; CELLS ; CHLORSULFURON |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000228017000010 |
| 公开日期 | 2013-10-25 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4163]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.NE Agr Univ, Res Ctr Life Sci & Biotechnol, Harbin 150030, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiang, WS,Wang, XJ,Ren, TR,et al. Expression of a wheat cytochrome P450 monooxygenase in yeast and its inhibition by glyphosate[J]. PEST MANAGEMENT SCIENCE,2005,61(4):402-406. |
| APA | Xiang, WS,Wang, XJ,Ren, TR,&Ju, XL.(2005).Expression of a wheat cytochrome P450 monooxygenase in yeast and its inhibition by glyphosate.PEST MANAGEMENT SCIENCE,61(4),402-406. |
| MLA | Xiang, WS,et al."Expression of a wheat cytochrome P450 monooxygenase in yeast and its inhibition by glyphosate".PEST MANAGEMENT SCIENCE 61.4(2005):402-406. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。