Immobilization of lipase onto micron-size magnetic beads
文献类型：期刊论文
| 作者 | Liu, XQ; Guan, YP; Shen, R; Liu, HZ |
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2005-08-05 |
| 卷号 | 822期号:1-2页码:91-97 |
| 关键词 | magnetic microsphere synthesis suspension polymerization Candida cylindracea lipase immobilization |
| ISSN号 | 1570-0232 |
| 其他题名 | J. Chromatogr. B |
| 中文摘要 | A novel and economical magnetic poly(methacrylate-divinylbenzene) microsphere (less than 8 mu m in diameter) was synthesized by the modified suspension polymerization of methacrylate and cross-linker divinylbenzene in the presence of magnetic fluid. Then, surface aminolysis was employed to obtain a high content of surface amino groups (0.40-0.55 mmol g(-1) supports). The morphology and properties of these magnetic supports were characterized with scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy and a vibrating sample magnetometer. These magnetic supports exhibited superparamagnetism with a high specific saturation magnetization (sigma(s)) of 14.6 emu g(-1). Candida cylindracea lipase was covalently immobilized on the amino-functionalized magnetic supports with the activity recovery up to 72.4% and enzyme loading of 34.0 mg g(-1) support, remarkably higher than the previous studies. The factors involved in the activity recovery and enzymatic properties of the immobilized lipase prepared were studied in comparison with free lipase, for which olive oil was chosen as the substrate. The results show that the immobilized lipase has good stability and reusability after recovery by magnetic separation within 20 s. (c) 2005 Elsevier B.V. All rights reserved. |
| 英文摘要 | A novel and economical magnetic poly(methacrylate-divinylbenzene) microsphere (less than 8 mu m in diameter) was synthesized by the modified suspension polymerization of methacrylate and cross-linker divinylbenzene in the presence of magnetic fluid. Then, surface aminolysis was employed to obtain a high content of surface amino groups (0.40-0.55 mmol g(-1) supports). The morphology and properties of these magnetic supports were characterized with scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy and a vibrating sample magnetometer. These magnetic supports exhibited superparamagnetism with a high specific saturation magnetization (sigma(s)) of 14.6 emu g(-1). Candida cylindracea lipase was covalently immobilized on the amino-functionalized magnetic supports with the activity recovery up to 72.4% and enzyme loading of 34.0 mg g(-1) support, remarkably higher than the previous studies. The factors involved in the activity recovery and enzymatic properties of the immobilized lipase prepared were studied in comparison with free lipase, for which olive oil was chosen as the substrate. The results show that the immobilized lipase has good stability and reusability after recovery by magnetic separation within 20 s. (c) 2005 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | POLYMER MICROSPHERES ; FATS ; NANOSPHERES ; ADSORPTION ; PARTICLES ; REACTORS ; CARRIERS ; ENZYME ; OILS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000231008900012 |
| 公开日期 | 2013-10-25 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4173]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Lab Separat Sci & Engn, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, XQ,Guan, YP,Shen, R,et al. Immobilization of lipase onto micron-size magnetic beads[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2005,822(1-2):91-97. |
| APA | Liu, XQ,Guan, YP,Shen, R,&Liu, HZ.(2005).Immobilization of lipase onto micron-size magnetic beads.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,822(1-2),91-97. |
| MLA | Liu, XQ,et al."Immobilization of lipase onto micron-size magnetic beads".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 822.1-2(2005):91-97. |
入库方式： OAI收割
来源：过程工程研究所
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