Improvement of NaNO2-oxidizing activity in Nitrobacter vulgaris by coentrapment in polyacrylamide containing polydimethylsiloxane copolymer and DEAE-sephadex
文献类型：期刊论文
| 作者 | Zhang, SP; Norrlow, O; Dey, ES |
| 刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2005-10-01 |
| 卷号 | 71期号:10页码:5888-5892 |
| 关键词 | agilis cells immobilized cells substrate consumption calcium alginate activated-sludge oxygen carriers nitrite model nitrification carrageenan |
| ISSN号 | 0099-2240 |
| 其他题名 | Appl. Environ. Microbiol. |
| 中文摘要 | Removal of nitrite and nitrate from drinking water has attracted great attention in recent years because of the human health risk induced by the exposure to contaminated groundwater and surface water. We have therefore tested a model nitrite oxidation system by coentrapping the NaNO2 oxidizer Nitrobacter vulgaris with polydimethylsiloxane (PDMS) copolymer and DEAE-Sephadex in a polyacrylamide gel. The copolymer and the anion exchanger facilitate the diffusion of oxygen and NaNO2, respectively, into the gel matrix. To test the nitrite-oxidizing activity, the entrapped cells were coupled to a thermal sensor. Coentrapment of 5% (wt/vol) DEAE-Sephadex with Nitrobacter vulgaris increased the nitrite-oxidizing activity by a factor of 3.7 compared to entrapped cells alone, and by the addition of 0.86% (wt/vol) artificial oxygen carrier PDMS copolymer increased the activity further to 4.3 times higher. Operational and storage stability of the coentrapped N. vulgaris also improved. This suggests that this enhanced immobilized cell system can also be used for nitrite oxidation to nitrate in drinking water as an on-line thermally monitored bioreactor. |
| 英文摘要 | Removal of nitrite and nitrate from drinking water has attracted great attention in recent years because of the human health risk induced by the exposure to contaminated groundwater and surface water. We have therefore tested a model nitrite oxidation system by coentrapping the NaNO2 oxidizer Nitrobacter vulgaris with polydimethylsiloxane (PDMS) copolymer and DEAE-Sephadex in a polyacrylamide gel. The copolymer and the anion exchanger facilitate the diffusion of oxygen and NaNO2, respectively, into the gel matrix. To test the nitrite-oxidizing activity, the entrapped cells were coupled to a thermal sensor. Coentrapment of 5% (wt/vol) DEAE-Sephadex with Nitrobacter vulgaris increased the nitrite-oxidizing activity by a factor of 3.7 compared to entrapped cells alone, and by the addition of 0.86% (wt/vol) artificial oxygen carrier PDMS copolymer increased the activity further to 4.3 times higher. Operational and storage stability of the coentrapped N. vulgaris also improved. This suggests that this enhanced immobilized cell system can also be used for nitrite oxidation to nitrate in drinking water as an on-line thermally monitored bioreactor. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 关键词[WOS] | AGILIS CELLS ; IMMOBILIZED CELLS ; SUBSTRATE CONSUMPTION ; CALCIUM ALGINATE ; ACTIVATED-SLUDGE ; OXYGEN CARRIERS ; NITRITE ; MODEL ; NITRIFICATION ; CARRAGEENAN |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000232504000028 |
| 公开日期 | 2013-10-25 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4176]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Lund Univ, S-22100 Lund, Sweden 2.Kemira Kemi AB, Recycling Comptence Ctr, S-25109 Helsingborg, Sweden 3.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, SP,Norrlow, O,Dey, ES. Improvement of NaNO2-oxidizing activity in Nitrobacter vulgaris by coentrapment in polyacrylamide containing polydimethylsiloxane copolymer and DEAE-sephadex[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2005,71(10):5888-5892. |
| APA | Zhang, SP,Norrlow, O,&Dey, ES.(2005).Improvement of NaNO2-oxidizing activity in Nitrobacter vulgaris by coentrapment in polyacrylamide containing polydimethylsiloxane copolymer and DEAE-sephadex.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,71(10),5888-5892. |
| MLA | Zhang, SP,et al."Improvement of NaNO2-oxidizing activity in Nitrobacter vulgaris by coentrapment in polyacrylamide containing polydimethylsiloxane copolymer and DEAE-sephadex".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 71.10(2005):5888-5892. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。