Ion-exchange chromatography of hepatitis B virus surface antigen from a recombinant Chinese hamster ovary cell line
文献类型：期刊论文
| 作者 | Zhou, WB; Bi, JX; Janson, JC; Dong, AH; Li, Y; Zhang, Y; Huang, YD; Su, ZG |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2005-11-18 |
| 卷号 | 1095期号:1-2页码:119-125 |
| 关键词 | hepatitis B surface antigen ion-exchange chromatography poly(ethylene glycol) retention behaviour |
| ISSN号 | 0021-9673 |
| 其他题名 | J. Chromatogr. A |
| 中文摘要 | About 10% of the Chinese population are chronic carriers of hepatitis B virus (HBV). Thus, the development of a highly efficient process for the preparation of a vaccine based on a recombinant hepatitis B surface antigen (HBsAg) is very important to the Chinese national immunization program. To this end, the ion exchange chromatography recovery of CHO-HBsAg from a recombinant Chinese hamster ovary cell line was shown to increase from about 55 to 80% by the addition of 1 % poly(ethylene glycol) (PEG 10,000) to the mobile phase. Furthermore, based on analysis by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), the intact glycoprotein form of CHO-HBsAg was completely preserved by the addition of PEG. In the absence of PEG the glycoprotein form of CHO-HBsAg was also spread out into the high salt elution fraction. High-performance size-exclusion chromatography with on-line multiangle-laser-light scattering (HPSEC-MALLS) analysis was performed to monitor the status of the CHO-HBsAg aggregate structure assembly, particle size and molecular weight distribution after each purification step, and the results showed further that the presence of PEG facilitated the separation and recovery of intact glycoprotein form of CHO-HBsAg and promoted their assembly to proper virus-like particles, which are both important features and prerequisites of their immunogenicity. (c) 2005 Elsevier B.V. All rights reserved. |
| 英文摘要 | About 10% of the Chinese population are chronic carriers of hepatitis B virus (HBV). Thus, the development of a highly efficient process for the preparation of a vaccine based on a recombinant hepatitis B surface antigen (HBsAg) is very important to the Chinese national immunization program. To this end, the ion exchange chromatography recovery of CHO-HBsAg from a recombinant Chinese hamster ovary cell line was shown to increase from about 55 to 80% by the addition of 1 % poly(ethylene glycol) (PEG 10,000) to the mobile phase. Furthermore, based on analysis by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), the intact glycoprotein form of CHO-HBsAg was completely preserved by the addition of PEG. In the absence of PEG the glycoprotein form of CHO-HBsAg was also spread out into the high salt elution fraction. High-performance size-exclusion chromatography with on-line multiangle-laser-light scattering (HPSEC-MALLS) analysis was performed to monitor the status of the CHO-HBsAg aggregate structure assembly, particle size and molecular weight distribution after each purification step, and the results showed further that the presence of PEG facilitated the separation and recovery of intact glycoprotein form of CHO-HBsAg and promoted their assembly to proper virus-like particles, which are both important features and prerequisites of their immunogenicity. (c) 2005 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | SIZE-EXCLUSION CHROMATOGRAPHY ; PARTICLES ; EXPRESSION ; PROTEINS ; SERUM ; YEAST ; DNA ; IMMUNOGENICITY ; PURIFICATION ; VACCINE |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000233411600015 |
| 公开日期 | 2013-10-25 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4182]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Uppsala Univ, Dept Surface Biotechnol, S-75123 Uppsala, Sweden 3.N China Pharmaceut Grp Corp, Shijiazhuang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, WB,Bi, JX,Janson, JC,et al. Ion-exchange chromatography of hepatitis B virus surface antigen from a recombinant Chinese hamster ovary cell line[J]. JOURNAL OF CHROMATOGRAPHY A,2005,1095(1-2):119-125. |
| APA | Zhou, WB.,Bi, JX.,Janson, JC.,Dong, AH.,Li, Y.,...&Su, ZG.(2005).Ion-exchange chromatography of hepatitis B virus surface antigen from a recombinant Chinese hamster ovary cell line.JOURNAL OF CHROMATOGRAPHY A,1095(1-2),119-125. |
| MLA | Zhou, WB,et al."Ion-exchange chromatography of hepatitis B virus surface antigen from a recombinant Chinese hamster ovary cell line".JOURNAL OF CHROMATOGRAPHY A 1095.1-2(2005):119-125. |
入库方式： OAI收割
来源：过程工程研究所
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