Novel polyethylene glycol derivative suitable for the preparation of mono-PEGylated protein
文献类型：期刊论文
| 作者 | Yun, Q; Chen, T; Zhang, GF; Bi, JX; Ma, GH; Su, ZG |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2005-02-01 |
| 卷号 | 27期号:3页码:213-217 |
| 关键词 | consensus interferon granulocyte-colony stimulating factor PEGylation polyethylene glycol |
| ISSN号 | 0141-5492 |
| 其他题名 | Biotechnol. Lett. |
| 中文摘要 | A novel methoxypolyethylene glycol (mPEG) derivative, containing a reactive group of 1-methyl pyridinium toluene-4-sulfonate, was synthesized and characterized. The mPEG derivative was successfully conjugated with two proteins: recombinant human granulocyte-colony stimulating factor (rhG-CSF) and consensus interferon (C-IFN). Homogeneous mono-PEGylated proteins were obtained which were identified by high performance size-exclusion chromatography and MALDI-TOF mass spectrometry. The biological activities of the mono-PEGylated rhG-CSF and the mono-PEGylated C-IFN were maintained at 90% and 88%, respectively. |
| 英文摘要 | A novel methoxypolyethylene glycol (mPEG) derivative, containing a reactive group of 1-methyl pyridinium toluene-4-sulfonate, was synthesized and characterized. The mPEG derivative was successfully conjugated with two proteins: recombinant human granulocyte-colony stimulating factor (rhG-CSF) and consensus interferon (C-IFN). Homogeneous mono-PEGylated proteins were obtained which were identified by high performance size-exclusion chromatography and MALDI-TOF mass spectrometry. The biological activities of the mono-PEGylated rhG-CSF and the mono-PEGylated C-IFN were maintained at 90% and 88%, respectively. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | PEPTIDE ; INTERFERON |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000227161100012 |
| 公开日期 | 2013-10-25 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4208]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Lab Biochem Engn, Beijing 100080, Peoples R China 2.Univ Sci & Technol Beijing, Sch Civil & Environm Engn, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yun, Q,Chen, T,Zhang, GF,et al. Novel polyethylene glycol derivative suitable for the preparation of mono-PEGylated protein[J]. BIOTECHNOLOGY LETTERS,2005,27(3):213-217. |
| APA | Yun, Q,Chen, T,Zhang, GF,Bi, JX,Ma, GH,&Su, ZG.(2005).Novel polyethylene glycol derivative suitable for the preparation of mono-PEGylated protein.BIOTECHNOLOGY LETTERS,27(3),213-217. |
| MLA | Yun, Q,et al."Novel polyethylene glycol derivative suitable for the preparation of mono-PEGylated protein".BIOTECHNOLOGY LETTERS 27.3(2005):213-217. |
入库方式： OAI收割
来源：过程工程研究所
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