One-step purification of alpha-amylase from the cultivation supernatant of recombinant Bacillus subtilis by high-speed counter-current chromatography with aqueous polymer two-phase systems
文献类型：期刊论文
| 作者 | Zhi, WB; Deng, QY; Song, JG; Gu, M; Ouyang, F |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2005-04-08 |
| 卷号 | 1070期号:1-2页码:215-219 |
| 关键词 | high-speed counter-current chromatography aqueous polymer two-phase system alpha-amylase purification |
| ISSN号 | 0021-9673 |
| 其他题名 | J. Chromatogr. A |
| 中文摘要 | Purification of a.-amylase from the cultivation supernatant of recombinant Bacillus subtilis by high-speed counter-current chromatography (HSCCC) in polyethylene glycol (PEG) 4000-inorganic salt aqueous polymer two-phase systems was studied. The effects of sodium chloride concentration on the partition coefficients of alpha-amylase and total protein were respectively tested in PEG4000-phosphate and PEG4000-citrate aqueous polymer two-phase systems to find the proper range of sodium chloride concentration for the HSCCC purification of alpha-amylase. alpha-Amylase was purified from the cultivation supernatant by HSCCC in PEG4000-phosphate system containing 2% (w/w) sodium chloride, yet with considerable loss of activity. PEG4000-citrate aqueous polymer two-phase system containing 2% (w/w) sodium chloride and supplemented with 0.56% (w/w) CaCl2 as protective agent was then successfully applied to purify a-amylase from cultivation supernatant by HSCCC to homogeneity and significantly increased the recovery of a-amylase activity from around 30 to 73.1%. (c) 2005 Elsevier B.V. All rights reserved. |
| 英文摘要 | Purification of a.-amylase from the cultivation supernatant of recombinant Bacillus subtilis by high-speed counter-current chromatography (HSCCC) in polyethylene glycol (PEG) 4000-inorganic salt aqueous polymer two-phase systems was studied. The effects of sodium chloride concentration on the partition coefficients of alpha-amylase and total protein were respectively tested in PEG4000-phosphate and PEG4000-citrate aqueous polymer two-phase systems to find the proper range of sodium chloride concentration for the HSCCC purification of alpha-amylase. alpha-Amylase was purified from the cultivation supernatant by HSCCC in PEG4000-phosphate system containing 2% (w/w) sodium chloride, yet with considerable loss of activity. PEG4000-citrate aqueous polymer two-phase system containing 2% (w/w) sodium chloride and supplemented with 0.56% (w/w) CaCl2 as protective agent was then successfully applied to purify a-amylase from cultivation supernatant by HSCCC to homogeneity and significantly increased the recovery of a-amylase activity from around 30 to 73.1%. (c) 2005 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | COIL PLANET CENTRIFUGE ; SEPARATION ; PARTITION ; PROTEINS ; BINDING ; LYSATE |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000228339200027 |
| 公开日期 | 2013-10-25 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4212]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China 2.Tauto Biotech, Shanghai 200122, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhi, WB,Deng, QY,Song, JG,et al. One-step purification of alpha-amylase from the cultivation supernatant of recombinant Bacillus subtilis by high-speed counter-current chromatography with aqueous polymer two-phase systems[J]. JOURNAL OF CHROMATOGRAPHY A,2005,1070(1-2):215-219. |
| APA | Zhi, WB,Deng, QY,Song, JG,Gu, M,&Ouyang, F.(2005).One-step purification of alpha-amylase from the cultivation supernatant of recombinant Bacillus subtilis by high-speed counter-current chromatography with aqueous polymer two-phase systems.JOURNAL OF CHROMATOGRAPHY A,1070(1-2),215-219. |
| MLA | Zhi, WB,et al."One-step purification of alpha-amylase from the cultivation supernatant of recombinant Bacillus subtilis by high-speed counter-current chromatography with aqueous polymer two-phase systems".JOURNAL OF CHROMATOGRAPHY A 1070.1-2(2005):215-219. |
入库方式： OAI收割
来源：过程工程研究所
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