One-step purification of epigallocatechin gallate from crude green tea extracts by isocratic hydrogen bond adsorption chromatography on beta-cyclodextrin substituted agarose gel media
文献类型：期刊论文
| 作者 | Xu, J; Zhang, GF; Tan, TW; Janson, JC |
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2005-09-25 |
| 卷号 | 824期号:1-2页码:323-326 |
| 关键词 | beta-cyclodextrin tea polyphenols EGCG catechin |
| ISSN号 | 1570-0232 |
| 其他题名 | J. Chromatogr. B |
| 中文摘要 | An oligomerized beta-cyclodextrin ligand coupled to brominated allyl-group substituted Sepharose HP has been used for the one-step purification of polyphenolic epigallocatechin gallate (EGCG), an important antioxidant, by isocratic hydrogen bond adsorption chromatography. With a sample load of 1.33 mg crude green tea polyphenolic extract per ml column packing and with water/ethanol/acetonitrile (57/30/13, v/v) as the optimum mobile phase, an EGCG purity of about 98% with a recovery of approximate 73% could be achieved by proper peak cutting. After about 10 sample applications, the column performance started to deteriorate but could be regenerated to its original function by cleaning with 0.35 M NaOH. (c) 2005 Elsevier B.V. All rights reserved. |
| 英文摘要 | An oligomerized beta-cyclodextrin ligand coupled to brominated allyl-group substituted Sepharose HP has been used for the one-step purification of polyphenolic epigallocatechin gallate (EGCG), an important antioxidant, by isocratic hydrogen bond adsorption chromatography. With a sample load of 1.33 mg crude green tea polyphenolic extract per ml column packing and with water/ethanol/acetonitrile (57/30/13, v/v) as the optimum mobile phase, an EGCG purity of about 98% with a recovery of approximate 73% could be achieved by proper peak cutting. After about 10 sample applications, the column performance started to deteriorate but could be regenerated to its original function by cleaning with 0.35 M NaOH. (c) 2005 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | LIQUID-CHROMATOGRAPHY ; STATIONARY-PHASE ; CELLS ; SEPARATION |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000231768900041 |
| 公开日期 | 2013-10-25 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4213]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Uppsala Univ, Dept Surface Biotechnol, Uppsala Biomed Ctr, SE-75123 Uppsala, Sweden 2.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China 3.Beijing Univ Chem Technol, Coll Life Sci & Technol, Dept Bioengn, Beijing 100029, Peoples R China 4.Uppsala Univ, Dept Surface Biotechnol, Uppsala Biomed Ctr, SE-75123 Uppsala, Sweden 5.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China 6.Beijing Univ Chem Technol, Coll Life Sci & Technol, Dept Bioengn, Beijing 100029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, J,Zhang, GF,Tan, TW,et al. One-step purification of epigallocatechin gallate from crude green tea extracts by isocratic hydrogen bond adsorption chromatography on beta-cyclodextrin substituted agarose gel media[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2005,824(1-2):323-326. |
| APA | Xu, J,Zhang, GF,Tan, TW,&Janson, JC.(2005).One-step purification of epigallocatechin gallate from crude green tea extracts by isocratic hydrogen bond adsorption chromatography on beta-cyclodextrin substituted agarose gel media.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,824(1-2),323-326. |
| MLA | Xu, J,et al."One-step purification of epigallocatechin gallate from crude green tea extracts by isocratic hydrogen bond adsorption chromatography on beta-cyclodextrin substituted agarose gel media".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 824.1-2(2005):323-326. |
入库方式： OAI收割
来源：过程工程研究所
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