Optimization of nutritional conditions for nattokinase production by Bacillus natto NLSSE using statistical experimental methods
文献类型：期刊论文
| 作者 | Liu, JG; Xing, JM; Chang, TS; Ma, ZY; Liu, HZ |
| 刊名 | PROCESS BIOCHEMISTRY
 |
| 出版日期 | 2005-07-01 |
| 卷号 | 40期号:8页码:2757-2762 |
| 关键词 | nattokinase Bacillus natto medium optimization statistical experiment methods (SEM) |
| ISSN号 | 1359-5113 |
| 其他题名 | Process Biochem. |
| 中文摘要 | Nattokinase is a potent fibrinolytic enzyme. The composition of the medium for the production of nattokinase was optimized with a stepwise strategy. Effects of various nitrogen and carbon sources were investigated. Fractional factorial design (FFD) was applied to elucidate the key ingredients in the media and the results indicated that the soy peptone, yeast extract and calcium chloride have a significant effect on nattokinase production. Central composite design (CCD) was employed to search for the optimal concentration of the three key components and the experimental results were fitted with a second-order polynomial model at 98% level (P < 0.02). Canonical analysis results showed that the response surface had a maximal point. According to the model, the predicted maximal nattokinase volumetric concentration was about 1273.8 units/ml and the corresponding concentration of soy peptone, calcium chloride and yeast extract were around 8.28, 0.64 and 0.74 g/l, respectively. The trial checking the developed medium showed a high nattokinase activity of 1300 60 units/ml. (c) 2005 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Nattokinase is a potent fibrinolytic enzyme. The composition of the medium for the production of nattokinase was optimized with a stepwise strategy. Effects of various nitrogen and carbon sources were investigated. Fractional factorial design (FFD) was applied to elucidate the key ingredients in the media and the results indicated that the soy peptone, yeast extract and calcium chloride have a significant effect on nattokinase production. Central composite design (CCD) was employed to search for the optimal concentration of the three key components and the experimental results were fitted with a second-order polynomial model at 98% level (P < 0.02). Canonical analysis results showed that the response surface had a maximal point. According to the model, the predicted maximal nattokinase volumetric concentration was about 1273.8 units/ml and the corresponding concentration of soy peptone, calcium chloride and yeast extract were around 8.28, 0.64 and 0.74 g/l, respectively. The trial checking the developed medium showed a high nattokinase activity of 1300 60 units/ml. (c) 2005 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | FIBRINOLYTIC ENZYME NATTOKINASE ; VEGETABLE CHEESE NATTO ; FERMENTATION ; PURIFICATION ; SUBTILIS ; FOOD ; RAT |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000229047600026 |
| 公开日期 | 2013-10-25 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4214]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Lab Separat Sci & Engn, Beijing 100080, Peoples R China 2.Hebei Univ Sci & Technol, Dept Biosci & Bioengn, Shijiazhuang 050018, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, JG,Xing, JM,Chang, TS,et al. Optimization of nutritional conditions for nattokinase production by Bacillus natto NLSSE using statistical experimental methods[J]. PROCESS BIOCHEMISTRY,2005,40(8):2757-2762. |
| APA | Liu, JG,Xing, JM,Chang, TS,Ma, ZY,&Liu, HZ.(2005).Optimization of nutritional conditions for nattokinase production by Bacillus natto NLSSE using statistical experimental methods.PROCESS BIOCHEMISTRY,40(8),2757-2762. |
| MLA | Liu, JG,et al."Optimization of nutritional conditions for nattokinase production by Bacillus natto NLSSE using statistical experimental methods".PROCESS BIOCHEMISTRY 40.8(2005):2757-2762. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。