Polystyrene-type uniform porous microsphere enables high resolution and low-pressure chromatography of natural products - A case study with icariin purification
文献类型：期刊论文
| 作者 | Sun, HH; Li, XN; Ma, GH; Su, ZG |
| 刊名 | CHROMATOGRAPHIA
 |
| 出版日期 | 2005 |
| 卷号 | 61期号:1-2页码:9-15 |
| 关键词 | column liquid chromatography icariin membrane emulsification uniform porous microsphere |
| ISSN号 | 0009-5893 |
| 其他题名 | Chromatographia |
| 中文摘要 | An efficient low-pressure preparative chromatography for purification of icariin from crude extract has been developed by employing a novel uniform porous microsphere, named PST, as packing material. A comparison was made between the PST medium and a commercially available silica-based C-18 medium on their column chromatographic performance. The PST column demonstrated a higher resolution and better selectivity than the C-18 column. Crude extract of 20% icariin can be purified to 90.0% with the recovery of 99.9% and column loadability of 2.5 mg-mL(-1) medium on the PST column under the optimized condition. After crystallization the purity of icariin can reach more than 98% with a total recovery of 93%. The product structure was determined by means of UV, IR, MS and NMR. |
| 英文摘要 | An efficient low-pressure preparative chromatography for purification of icariin from crude extract has been developed by employing a novel uniform porous microsphere, named PST, as packing material. A comparison was made between the PST medium and a commercially available silica-based C-18 medium on their column chromatographic performance. The PST column demonstrated a higher resolution and better selectivity than the C-18 column. Crude extract of 20% icariin can be purified to 90.0% with the recovery of 99.9% and column loadability of 2.5 mg-mL(-1) medium on the PST column under the optimized condition. After crystallization the purity of icariin can reach more than 98% with a total recovery of 93%. The product structure was determined by means of UV, IR, MS and NMR. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | ANTI-HYPOXIA/REOXYGENATION AGENTS ; LIQUID-CHROMATOGRAPHY ; FLAVONOL GLYCOSIDES ; RETENTION ; COLUMNS ; CELLS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000226506000002 |
| 公开日期 | 2013-10-25 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4224]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Beijing Inst Chem Technol, Sch Chem Engn, Beijing 100029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, HH,Li, XN,Ma, GH,et al. Polystyrene-type uniform porous microsphere enables high resolution and low-pressure chromatography of natural products - A case study with icariin purification[J]. CHROMATOGRAPHIA,2005,61(1-2):9-15. |
| APA | Sun, HH,Li, XN,Ma, GH,&Su, ZG.(2005).Polystyrene-type uniform porous microsphere enables high resolution and low-pressure chromatography of natural products - A case study with icariin purification.CHROMATOGRAPHIA,61(1-2),9-15. |
| MLA | Sun, HH,et al."Polystyrene-type uniform porous microsphere enables high resolution and low-pressure chromatography of natural products - A case study with icariin purification".CHROMATOGRAPHIA 61.1-2(2005):9-15. |
入库方式： OAI收割
来源：过程工程研究所
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