Preparation and characterization of micron-sized non-porous magnetic polymer microspheres with immobilized metal affinity ligands by modified suspension polymerization
文献类型：期刊论文
| 作者 | Ma, ZY; Guan, YP; Liu, XQ; Liu, HZ |
| 刊名 | JOURNAL OF APPLIED POLYMER SCIENCE
 |
| 出版日期 | 2005-06-15 |
| 卷号 | 96期号:6页码:2174-2180 |
| 关键词 | magnetic polymers copolymerization functionalization of polymers modified suspension polymerization immobilized metal affinity adsorption |
| ISSN号 | 0021-8995 |
| 其他题名 | J. Appl. Polym. Sci. |
| 中文摘要 | A novel preparation method of micron-sized non-porous magnetic polymer microspheres with immobilized metal affinity ligands was developed. A modified suspension polymerization of methacrylate (MA) and divinylbenzene (DVB) was performed in the presence of oleic acid-coated magnetic Fe3O4 nanoparticles to obtain magnetic poly (methacrylate-divinylbenzene) (mPMA-DVB) microspheres. Through ammonolysis using ethylenediamine (EDA) and subsequent carboxymethylation with chloroacetic acid, magnetic polymer microspheres with chelate ligands of iminodiacetic acid (IDA) were obtained. Charging with copper ions resulted in magnetic polymer microspheres capable of binding proteins that display metal affinity. The morphology, magnetic properties, and composition of magnetic polymer microspheres were characterized with scanning electron microscopy (SEM), vibrating sample magnetometer (VSM), and Fourier transform infrared spectroscopy (FTIR), respectively. Bovine hemoglobin (BHb) was adopted as a model protein to investigate their affinity adsorption capacity. It was found that the adsorption capacity was as high as 168.2 mg/g microspheres and with rather low non-specific adsorption. (c) 2005 Wiley Periodicals, Inc. |
| 英文摘要 | A novel preparation method of micron-sized non-porous magnetic polymer microspheres with immobilized metal affinity ligands was developed. A modified suspension polymerization of methacrylate (MA) and divinylbenzene (DVB) was performed in the presence of oleic acid-coated magnetic Fe3O4 nanoparticles to obtain magnetic poly (methacrylate-divinylbenzene) (mPMA-DVB) microspheres. Through ammonolysis using ethylenediamine (EDA) and subsequent carboxymethylation with chloroacetic acid, magnetic polymer microspheres with chelate ligands of iminodiacetic acid (IDA) were obtained. Charging with copper ions resulted in magnetic polymer microspheres capable of binding proteins that display metal affinity. The morphology, magnetic properties, and composition of magnetic polymer microspheres were characterized with scanning electron microscopy (SEM), vibrating sample magnetometer (VSM), and Fourier transform infrared spectroscopy (FTIR), respectively. Bovine hemoglobin (BHb) was adopted as a model protein to investigate their affinity adsorption capacity. It was found that the adsorption capacity was as high as 168.2 mg/g microspheres and with rather low non-specific adsorption. (c) 2005 Wiley Periodicals, Inc. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Polymer Science |
| 研究领域[WOS] | Polymer Science |
| 关键词[WOS] | PROTEIN FRACTIONATION ; ADSORPTION ; CHROMATOGRAPHY ; SUPPORTS ; MATRIX ; BEADS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000228776100023 |
| 公开日期 | 2013-10-25 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4230]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Lab Separat Sci & Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, ZY,Guan, YP,Liu, XQ,et al. Preparation and characterization of micron-sized non-porous magnetic polymer microspheres with immobilized metal affinity ligands by modified suspension polymerization[J]. JOURNAL OF APPLIED POLYMER SCIENCE,2005,96(6):2174-2180. |
| APA | Ma, ZY,Guan, YP,Liu, XQ,&Liu, HZ.(2005).Preparation and characterization of micron-sized non-porous magnetic polymer microspheres with immobilized metal affinity ligands by modified suspension polymerization.JOURNAL OF APPLIED POLYMER SCIENCE,96(6),2174-2180. |
| MLA | Ma, ZY,et al."Preparation and characterization of micron-sized non-porous magnetic polymer microspheres with immobilized metal affinity ligands by modified suspension polymerization".JOURNAL OF APPLIED POLYMER SCIENCE 96.6(2005):2174-2180. |
入库方式： OAI收割
来源：过程工程研究所
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