Regulation of CO2 on heterocyst differentiation and nitrate uptake in the cyanobacterium Anabaena sp PCC 7120
文献类型：期刊论文
| 作者 | Kang, RJ; Shi, DJ; Cong, W; Cai, ZL; Ouyang, F |
| 刊名 | JOURNAL OF APPLIED MICROBIOLOGY
 |
| 出版日期 | 2005 |
| 卷号 | 98期号:3页码:693-698 |
| ISSN号 | 1364-5072 |
| 关键词 | Anabaena sp PCC7120 CO2 heterocyst differentiation nitrate uptake |
| 其他题名 | J. Appl. Microbiol. |
| 中文摘要 | Aims: The aim of the present investigation was to study the effects of different inorganic carbon and nitrogen sources on nitrate uptake and heterocyst differentiation in the culture of cyanobacterium Anabaena sp. PCC 7120. Methods and Results: Anabaena was cultivated in media BG11 containing combined nitrogen and supplementary NaHCO3 or CO2. Cell growth, heterocyst differentiation, nitrate reductase (NR, EC 1.7.7.2), glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) and NO3- uptake were analysed. The cells cultivated in BG11(0) medium with aeration were taken as reference. Experimental results showed that the differentiation frequency of heterocysts when the cells were cultivated with elevated CO2 was higher than that of the cells grown with air or bicarbonate. Heterocysts appeared unexpectedly when CO2 was introduced into the medium containing nitrate. However, no heterocysts emerged when CO2 was added to medium containing NH4+ or urea, or when NaHCO3 was supplied to the medium with nitrate. Both nitrate uptake rate and nitrate reduction enzyme activity were depressed by the supplement of CO2 to the culture. The activity of G6PDH was enhanced with the increase in heterocyst differentiation frequency. Conclusions: CO2 might compete with NO3- for energy and electrons in the uptake process and CO2 appears favoured. This led to a high intracellular C/N ratio and a relative N limitation. So the process of heterocyst differentiation was activated to supplement nitrogen uptake. Significance and Impact of the Study: This study provided an attractive possibility to form more heterocysts by rapid growth of Anabaena cells cultivated in the medium containing nitrate in order to increase nitrogen fixation and hydrogen production. |
| 英文摘要 | Aims: The aim of the present investigation was to study the effects of different inorganic carbon and nitrogen sources on nitrate uptake and heterocyst differentiation in the culture of cyanobacterium Anabaena sp. PCC 7120. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 关键词[WOS] | ELEVATED CARBON-DIOXIDE ; MARINE-PHYTOPLANKTON ; ANACYSTIS-NIDULANS ; ASSIMILATION ; NITROGENASE ; STRAIN ; DEHYDROGENASE ; CYLINDRICA ; VARIABILIS ; REDUCTASE |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000226963200019 |
| 公开日期 | 2013-10-25 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4244]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Kang, RJ,Shi, DJ,Cong, W,et al. Regulation of CO2 on heterocyst differentiation and nitrate uptake in the cyanobacterium Anabaena sp PCC 7120[J]. JOURNAL OF APPLIED MICROBIOLOGY,2005,98(3):693-698. |
| APA | Kang, RJ,Shi, DJ,Cong, W,Cai, ZL,&Ouyang, F.(2005).Regulation of CO2 on heterocyst differentiation and nitrate uptake in the cyanobacterium Anabaena sp PCC 7120.JOURNAL OF APPLIED MICROBIOLOGY,98(3),693-698. |
| MLA | Kang, RJ,et al."Regulation of CO2 on heterocyst differentiation and nitrate uptake in the cyanobacterium Anabaena sp PCC 7120".JOURNAL OF APPLIED MICROBIOLOGY 98.3(2005):693-698. |
入库方式： OAI收割
来源：过程工程研究所
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