Suitability of steric mass-action model for ion-exchange equilibrium of micromolecule
文献类型：期刊论文
| 作者 | Chen, WD; Wang, YD; Su, ZG |
| 刊名 | CHINESE JOURNAL OF CHEMICAL ENGINEERING
 |
| 出版日期 | 2005-02-01 |
| 卷号 | 13期号:1页码:18-23 |
| 关键词 | micromolecule ion-exchange equilibrium steric mass-action model chromatography |
| ISSN号 | 1004-9541 |
| 其他题名 | Chin. J. Chem. Eng. |
| 中文摘要 | The steric mass-action (SMA) model has been widely reported in the literature for ion-exchange and metal-affinity interaction adsorption equilibrium of biomacromolecules. In this paper, the usefulness of SMA model is analyzed for describing micromolecule ion-exchange equilibrium onto cation exchangers, CM Sephadex C-25 and Streamline SP. Batch adsorption experiments with ephedrine hydrochloride as a model adsorbate are carried out to determine the model parameters, that is, steric factor, characteristic charge and equilibrium constant. The result shows that the SMA model parameters of micromolecule cannot be obtained using the nonlinear least-square fitting method as protein's due to the remarkable difference between the molecular mass and dimension of micromolecule and protein. It is considered that the small size of the adsorbates dealt with in this study justifies the neglect of steric hindrances arising from adsorbate bulkiness. Thus, the three-parameter SMA model is reduced to two-parameter one (i.e., steric factor is equal to zero) for describing micromolecule ion-exchange equilibrium. It is found that the equilibrium constant for CM Sephadex C-25 increases with increasing ionic strength, while the equilibrium constant for Streamline SP shows an opposite trend. This is probably due to the remarkable difference between the physical properties of the two adsorbents. Then, the relationship between the equilibrium constant and ionic strength is described by an expression. The computer simulations show that, the theoretical model with the correlation is promising in the prediction of micromolecule adsorption decrease with increasing ionic strength in a wide range of salt concentration. |
| 英文摘要 | The steric mass-action (SMA) model has been widely reported in the literature for ion-exchange and metal-affinity interaction adsorption equilibrium of biomacromolecules. In this paper, the usefulness of SMA model is analyzed for describing micromolecule ion-exchange equilibrium onto cation exchangers, CM Sephadex C-25 and Streamline SP. Batch adsorption experiments with ephedrine hydrochloride as a model adsorbate are carried out to determine the model parameters, that is, steric factor, characteristic charge and equilibrium constant. The result shows that the SMA model parameters of micromolecule cannot be obtained using the nonlinear least-square fitting method as protein's due to the remarkable difference between the molecular mass and dimension of micromolecule and protein. It is considered that the small size of the adsorbates dealt with in this study justifies the neglect of steric hindrances arising from adsorbate bulkiness. Thus, the three-parameter SMA model is reduced to two-parameter one (i.e., steric factor is equal to zero) for describing micromolecule ion-exchange equilibrium. It is found that the equilibrium constant for CM Sephadex C-25 increases with increasing ionic strength, while the equilibrium constant for Streamline SP shows an opposite trend. This is probably due to the remarkable difference between the physical properties of the two adsorbents. Then, the relationship between the equilibrium constant and ionic strength is described by an expression. The computer simulations show that, the theoretical model with the correlation is promising in the prediction of micromolecule adsorption decrease with increasing ionic strength in a wide range of salt concentration. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Chemical |
| 研究领域[WOS] | Engineering |
| 关键词[WOS] | EXPANDED BED ADSORPTION ; CHINENSIS VAR. MAIREI ; PROTEIN ADSORPTION ; SUSPENSION-CULTURES ; CHROMATOGRAPHY ; RETENTION ; DEPENDENCE ; ADSORBENT ; ELUTION |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000227861600004 |
| 公开日期 | 2013-10-25 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4263]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Univ Petr, Sch Chem Sci & Engn, Dept Biochem Engn, Beijing 102249, Peoples R China 2.GSF, Inst Mol Radiobiol, D-85764 Neuherberg, Germany 3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, WD,Wang, YD,Su, ZG. Suitability of steric mass-action model for ion-exchange equilibrium of micromolecule[J]. CHINESE JOURNAL OF CHEMICAL ENGINEERING,2005,13(1):18-23. |
| APA | Chen, WD,Wang, YD,&Su, ZG.(2005).Suitability of steric mass-action model for ion-exchange equilibrium of micromolecule.CHINESE JOURNAL OF CHEMICAL ENGINEERING,13(1),18-23. |
| MLA | Chen, WD,et al."Suitability of steric mass-action model for ion-exchange equilibrium of micromolecule".CHINESE JOURNAL OF CHEMICAL ENGINEERING 13.1(2005):18-23. |
入库方式： OAI收割
来源：过程工程研究所
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