Synthesis of monodisperse nonporous crosslinked poly(glycidyl methacrylate) particles with metal affinity ligands for protein adsorption
文献类型：期刊论文
| 作者 | Ma, ZY; Guan, YP; Liu, HZ |
| 刊名 | POLYMER INTERNATIONAL
 |
| 出版日期 | 2005-11-01 |
| 卷号 | 54期号:11页码:1502-1507 |
| 关键词 | crosslinked monodisperse particles nonporous dispersion polymerization poly(glycidyl methacrylate) metal affinity separation protein adsorption |
| ISSN号 | 0959-8103 |
| 其他题名 | Polym. Int. |
| 中文摘要 | Monodisperse nonporous crosslinked poly(glycidyl methacrylate) (PGMA) particles with immobilized metal affinity ligands were prepared for selective recovery of proteins. The PGMA particles, with an average size of 2.2 mu m, were prepared by a simple dispersion polymerization of glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EGDMA). The particles were characterized by scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR). The epoxy groups of the particles were modified with the metal chelating agent iminodiacetic acid (IDA), which forms metal-IDA chelates at the active sites. After charging with copper ions, the particles were used to recover a model protein, bovine hemoglobin (BHb), in a batchwise manner. The particles had the adsorption capacity of 218.7 mg g(-1) with little nonspecific adsorption. The adsorption behavior could be described with the Langmuir equation. The effect of pH on the adsorption was also studied. Regeneration of the metal-chelated particles was easily performed with 50 mmol L-1 ethylenediaminetetraacetic acid (EDTA), followed by washing with water and reloading with Cu2+. The particles could be very useful as an affinity separation adsorbent. (c) 2005 Society of Chemical Industry. |
| 英文摘要 | Monodisperse nonporous crosslinked poly(glycidyl methacrylate) (PGMA) particles with immobilized metal affinity ligands were prepared for selective recovery of proteins. The PGMA particles, with an average size of 2.2 mu m, were prepared by a simple dispersion polymerization of glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EGDMA). The particles were characterized by scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR). The epoxy groups of the particles were modified with the metal chelating agent iminodiacetic acid (IDA), which forms metal-IDA chelates at the active sites. After charging with copper ions, the particles were used to recover a model protein, bovine hemoglobin (BHb), in a batchwise manner. The particles had the adsorption capacity of 218.7 mg g(-1) with little nonspecific adsorption. The adsorption behavior could be described with the Langmuir equation. The effect of pH on the adsorption was also studied. Regeneration of the metal-chelated particles was easily performed with 50 mmol L-1 ethylenediaminetetraacetic acid (EDTA), followed by washing with water and reloading with Cu2+. The particles could be very useful as an affinity separation adsorbent. (c) 2005 Society of Chemical Industry. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Polymer Science |
| 研究领域[WOS] | Polymer Science |
| 关键词[WOS] | DISPERSION COPOLYMERIZATION ; GLYCIDYL METHACRYLATE ; METHYL-METHACRYLATE ; POLYMER PARTICLES ; CHROMATOGRAPHY ; SEPARATION ; SIZE ; MICROSPHERES ; PURIFICATION ; SORBENTS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000232591700006 |
| 公开日期 | 2013-10-25 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4271]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Lab Separat Sci & Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, ZY,Guan, YP,Liu, HZ. Synthesis of monodisperse nonporous crosslinked poly(glycidyl methacrylate) particles with metal affinity ligands for protein adsorption[J]. POLYMER INTERNATIONAL,2005,54(11):1502-1507. |
| APA | Ma, ZY,Guan, YP,&Liu, HZ.(2005).Synthesis of monodisperse nonporous crosslinked poly(glycidyl methacrylate) particles with metal affinity ligands for protein adsorption.POLYMER INTERNATIONAL,54(11),1502-1507. |
| MLA | Ma, ZY,et al."Synthesis of monodisperse nonporous crosslinked poly(glycidyl methacrylate) particles with metal affinity ligands for protein adsorption".POLYMER INTERNATIONAL 54.11(2005):1502-1507. |
入库方式： OAI收割
来源：过程工程研究所
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