Glycerol-Assisted Hydrophobic Interaction Chromatography Improving Refolding of Recombinant Human Granulocyte Colony-Stimulating Factor
文献类型：期刊论文
| 作者 | Wang, Fangwei1,2; Liu, Yongdong1; Ma, Guanghui1; Su, Zhiguo1 |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 159期号:3页码:634-641 |
| 关键词 | Hydrophobic interaction chromatography Refolding Glycerol Recombinant human granulocyte colony-stimulating factor |
| ISSN号 | 0273-2289 |
| 英文摘要 | Efficient refolding of recombinant proteins in the forms of inclusion bodies at higher concentration remains challenging. Here, we report a strategy of a dual-gradient hydrophobic interaction chromatography (HIC) mode to refold recombinant human granulocyte colony-stimulating factor from its inclusion bodies at high protein concentration. The strategy was taken to meet the demand of dynamic refolding proceeding by gradually decrease the denaturant (guanidine-HCl) concentration and gradually increase the hydrophilicity of media (column of Poros PE 20) with glycerol as additive to provide a mild refolding surroundings. Compared with dilution method, this dual-gradient HIC process gave about 8.5-fold of increase in specific activity and 30% increase in soluble protein recovery. Furthermore, much higher protein concentration could be obtained at the same time. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | ESCHERICHIA-COLI ; DENATURED/REDUCED LYSOZYME ; INCLUSION-BODIES ; PROTEINS ; RENATURATION ; INTERFERON ; GRADIENTS |
| 收录类别 | SCI ; ISTP |
| 语种 | 英语 |
| WOS记录号 | WOS:000271068000006 |
| 公开日期 | 2013-10-29 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4362]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China 2.Dalian Med Univ, Clin Coll 2, Dalian 116027, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Fangwei,Liu, Yongdong,Ma, Guanghui,et al. Glycerol-Assisted Hydrophobic Interaction Chromatography Improving Refolding of Recombinant Human Granulocyte Colony-Stimulating Factor[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2009,159(3):634-641. |
| APA | Wang, Fangwei,Liu, Yongdong,Ma, Guanghui,&Su, Zhiguo.(2009).Glycerol-Assisted Hydrophobic Interaction Chromatography Improving Refolding of Recombinant Human Granulocyte Colony-Stimulating Factor.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,159(3),634-641. |
| MLA | Wang, Fangwei,et al."Glycerol-Assisted Hydrophobic Interaction Chromatography Improving Refolding of Recombinant Human Granulocyte Colony-Stimulating Factor".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 159.3(2009):634-641. |
入库方式： OAI收割
来源：过程工程研究所
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