Artemisia judaica L.: micropropagation and antioxidant activity
文献类型：期刊论文
| 作者 | Liu, CZ; Murch, SJ; El-Demerdash, M; Saxena, PK |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2004-05-13 |
| 卷号 | 110期号:1页码:63-71 |
| 关键词 | Artemisia judaica L. Egyptian medicinal plant shoot culture bioreactor flavonoids antioxidant activity |
| ISSN号 | 0168-1656 |
| 其他题名 | J. Biotechnol. |
| 中文摘要 | Artemisia judaica L., an Egyptian medicinal plant used in the treatment of gastrointestinal disorders, was mass-propagated and grown using solid, paper-bridge-support liquid, liquid-flask and bioreactor cultures. The liquid-flask culture using 50 ml MS liquid medium in 250 ml flask yielded significantly greater shoot proliferation than either solid cultures or paper-bridge-support liquid cultures. Increasing flask capacity from 100 to 500 ml improved shoot proliferation and growth. Mass-propagation efficiencies of various bioreactor systems, viz. temporary immersion reactors and bubble column reactors, were also compared. The temporary immersion bioreactor was found to have significant advantages for A. judaica shoot proliferation. The shoot cultures from the temporary immersion reactor formed complete plantlets when subcultured onto a medium containing 1 mumol l(-1) indole-3-butyric acid (IBA), and mature plants were established, acclimatized and thrived in standard greenhouse conditions. Assays of antioxidant activity and total flavonoid content of in vitro and in vivo grown tissues were evaluated as gross parameters of medicinal efficacy. Significantly higher antioxidant activity and flavonoid contents were observed in the tissues of mature greenhouse-grown plants. The efficient in vitro production systems developed in this study provided sterile, consistent tissues for investigation of bioactivity and germplasm conservation of A. judaica. (C) 2004 Elsevier B.V. All rights reserved. |
| 英文摘要 | Artemisia judaica L., an Egyptian medicinal plant used in the treatment of gastrointestinal disorders, was mass-propagated and grown using solid, paper-bridge-support liquid, liquid-flask and bioreactor cultures. The liquid-flask culture using 50 ml MS liquid medium in 250 ml flask yielded significantly greater shoot proliferation than either solid cultures or paper-bridge-support liquid cultures. Increasing flask capacity from 100 to 500 ml improved shoot proliferation and growth. Mass-propagation efficiencies of various bioreactor systems, viz. temporary immersion reactors and bubble column reactors, were also compared. The temporary immersion bioreactor was found to have significant advantages for A. judaica shoot proliferation. The shoot cultures from the temporary immersion reactor formed complete plantlets when subcultured onto a medium containing 1 mumol l(-1) indole-3-butyric acid (IBA), and mature plants were established, acclimatized and thrived in standard greenhouse conditions. Assays of antioxidant activity and total flavonoid content of in vitro and in vivo grown tissues were evaluated as gross parameters of medicinal efficacy. Significantly higher antioxidant activity and flavonoid contents were observed in the tissues of mature greenhouse-grown plants. The efficient in vitro production systems developed in this study provided sterile, consistent tissues for investigation of bioactivity and germplasm conservation of A. judaica. (C) 2004 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | SUSPENSION CULTURES ; POLYURETHANE FOAM ; REGENERATION ; COMPONENTS ; BIOREACTOR ; MONOSPERMA ; CLUSTERS ; PLANTS ; CALLUS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000221492900007 |
| 公开日期 | 2013-11-05 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4898]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Univ Guelph, Div Biotechnol, Dept Plant Agr, Guelph, ON N1G 2W1, Canada 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 3.Natl Trop Bot Garden, Inst Ethnobot, Kalaheo, HI USA 4.Univ Mansoura, Fac Sci, Mansoura, Egypt |
| 推荐引用方式 GB/T 7714 | Liu, CZ,Murch, SJ,El-Demerdash, M,et al. Artemisia judaica L.: micropropagation and antioxidant activity[J]. JOURNAL OF BIOTECHNOLOGY,2004,110(1):63-71. |
| APA | Liu, CZ,Murch, SJ,El-Demerdash, M,&Saxena, PK.(2004).Artemisia judaica L.: micropropagation and antioxidant activity.JOURNAL OF BIOTECHNOLOGY,110(1),63-71. |
| MLA | Liu, CZ,et al."Artemisia judaica L.: micropropagation and antioxidant activity".JOURNAL OF BIOTECHNOLOGY 110.1(2004):63-71. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。