Cell culture of Saussurea medusa in a periodically submerged air-lift bioreactor
文献类型：期刊论文
| 作者 | Yuan, XF; Zhao, B; Wang, YC |
| 刊名 | BIOCHEMICAL ENGINEERING JOURNAL
 |
| 出版日期 | 2004-11-01 |
| 卷号 | 21期号:3页码:235-239 |
| 关键词 | bioreactor engineering flavonoids periodically submerged airlift bioreactor (PSAB) plant cell culture Saussurea medusa secondary metabolite |
| ISSN号 | 1369-703X |
| 其他题名 | Biochem. Eng. J. |
| 中文摘要 | Cell culture of Saussurea medusa in a 2-1 periodically submerged air-lift bioreactor (PSAB) was investigated. The periodically unsubmerged (immobilization culture) and submerged (suspension culture) operation of the whole bioreactor system was controlled by the electromagnetic valves and driven rod controller connected with the time controller. The maximum cell biomass (10.4 gl(-1)), content of total flavonoids (4.8%), production of total flavonoids (501 mgl(-1)) and the optimal size distribution of cell aggregates were obtained under the optimal unsubmerged/submerged period of 10 min/8 h (the cell inoculation size was 1.0 gl(-1), and the air flowrate was 20/401 h(-1)). Though the content of total flavonoids was still a little lower than that obtained from the shake flask culture, the specific growth rate of cells in PSAB was 2.1-fold and the production of total flavonoids was 1.7-fold of those in shake flasks. It was concluded that PSAB had advantages in improving cell growth of S. medusa and the production of total flavonoids. (C) 2004 Elsevier B.V. All rights reserved. |
| 英文摘要 | Cell culture of Saussurea medusa in a 2-1 periodically submerged air-lift bioreactor (PSAB) was investigated. The periodically unsubmerged (immobilization culture) and submerged (suspension culture) operation of the whole bioreactor system was controlled by the electromagnetic valves and driven rod controller connected with the time controller. The maximum cell biomass (10.4 gl(-1)), content of total flavonoids (4.8%), production of total flavonoids (501 mgl(-1)) and the optimal size distribution of cell aggregates were obtained under the optimal unsubmerged/submerged period of 10 min/8 h (the cell inoculation size was 1.0 gl(-1), and the air flowrate was 20/401 h(-1)). Though the content of total flavonoids was still a little lower than that obtained from the shake flask culture, the specific growth rate of cells in PSAB was 2.1-fold and the production of total flavonoids was 1.7-fold of those in shake flasks. It was concluded that PSAB had advantages in improving cell growth of S. medusa and the production of total flavonoids. (C) 2004 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000224295400006 |
| 公开日期 | 2013-11-05 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4905]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yuan, XF,Zhao, B,Wang, YC. Cell culture of Saussurea medusa in a periodically submerged air-lift bioreactor[J]. BIOCHEMICAL ENGINEERING JOURNAL,2004,21(3):235-239. |
| APA | Yuan, XF,Zhao, B,&Wang, YC.(2004).Cell culture of Saussurea medusa in a periodically submerged air-lift bioreactor.BIOCHEMICAL ENGINEERING JOURNAL,21(3),235-239. |
| MLA | Yuan, XF,et al."Cell culture of Saussurea medusa in a periodically submerged air-lift bioreactor".BIOCHEMICAL ENGINEERING JOURNAL 21.3(2004):235-239. |
入库方式： OAI收割
来源：过程工程研究所
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