CMCase activity assay as a method for cellulase adsorption analysis
文献类型：期刊论文
| 作者 | Zhou, XH; Chen, HZ ; Li, ZH
|
| 刊名 | ENZYME AND MICROBIAL TECHNOLOGY
 |
| 出版日期 | 2004-10-06 |
| 卷号 | 35期号:5页码:455-459 |
| 关键词 | CMCase activity assay filter paper activity assay protein concentration assay cellulase adsorption |
| ISSN号 | 0141-0229 |
| 其他题名 | Enzyme Microb. Technol. |
| 中文摘要 | Microcrystalline cellulose (MCC) was hydrolyzed with Trichoderma viride cellulase, linear correlation was found between free filter paper activity (FPA) and free carboxymethyl cellulase activity (CA), and between bound CA and bound protein concentration at 0-72 h reaction time. Linear correlation between bound FPA and bound protein concentration was found at short reaction time (I h), but not found at long reaction time. CA, the activity of endoglucanase, may be used to describe the adsorption of whole cellulase, at least, for MCC-T viride cellulase system. and the error for CA assay was less than which for FPA assay and protein concentration assay in hydrolysis condition. (C) 2004 Elsevier Inc. All rights reserved. |
| 英文摘要 | Microcrystalline cellulose (MCC) was hydrolyzed with Trichoderma viride cellulase, linear correlation was found between free filter paper activity (FPA) and free carboxymethyl cellulase activity (CA), and between bound CA and bound protein concentration at 0-72 h reaction time. Linear correlation between bound FPA and bound protein concentration was found at short reaction time (I h), but not found at long reaction time. CA, the activity of endoglucanase, may be used to describe the adsorption of whole cellulase, at least, for MCC-T viride cellulase system. and the error for CA assay was less than which for FPA assay and protein concentration assay in hydrolysis condition. (C) 2004 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | TRICHODERMA-REESEI ; HYDROLYSIS ; COMPONENTS ; SYNERGISM ; ACID |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000224142200013 |
| 公开日期 | 2013-11-05 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4908]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, XH,Chen, HZ,Li, ZH. CMCase activity assay as a method for cellulase adsorption analysis[J]. ENZYME AND MICROBIAL TECHNOLOGY,2004,35(5):455-459. |
| APA | Zhou, XH,Chen, HZ,&Li, ZH.(2004).CMCase activity assay as a method for cellulase adsorption analysis.ENZYME AND MICROBIAL TECHNOLOGY,35(5),455-459. |
| MLA | Zhou, XH,et al."CMCase activity assay as a method for cellulase adsorption analysis".ENZYME AND MICROBIAL TECHNOLOGY 35.5(2004):455-459. |
入库方式： OAI收割
来源：过程工程研究所
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