Goldenseal (Hydrastis canadensis L.): In vitro regeneration for germplasm conservation and elimination of heavy metal contamination
文献类型：期刊论文
| 作者 | Liu, CZ; Murch, SJ; Jain, JC; Saxena, PK |
| 刊名 | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
 |
| 出版日期 | 2004 |
| 卷号 | 40期号:1页码:75-79 |
| 关键词 | Hydrastis canadensis L medicinal plant plant regeneration heavy metal contamination |
| ISSN号 | 1054-5476 |
| 其他题名 | In Vitro Cell. Dev. Biol.-Plant |
| 中文摘要 | Hydrastis canadensis L. (Goldenseal) is an endangered medicinal plant used in the treatment of many ailments, such as gastrointestinal disturbances, urinary disorders, hemorrhage, skin, mouth and eye infections, and inflammation. Commercial preparations of wild-harvested goldenseal were found to contain heavy metal contaminants including aluminum (848 mug g(-1)), cadmium (0.4 mug g(-1)), lead (18.7 mug g(-1)), and mercury (0.1 mug g(-1)). As well, goldenseal is an endangered species listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) Appendix II. Therefore, the practice of wild-harvest is actually decimating natural populations of goldenseal and endangering its genetic diversity. In vitro propagation protocol by tissue culture was developed for producing high-quality tissues of goldenseal. Significantly more de novo, regeneration was induced on stem explants of 3-mo.-old plants cultured on a medium containing 10 muM 6-benzylaminopurine (BA) (22 regenerants per explant) than any other treatment. Subculture of the regenerants on a medium devoid of growth regulators resulted in the development of, complete plants that were acclimatized and thrived in standard greenhouse conditions. The plants regenerated in vitro contained the lowest levels of heavy metals. The findings of this study provide the first evidence that heavy metal contaminants bioaccumulate in goldenseal tissues and also provide a method for germplasm conservation, mass multiplication, and production of goldenseal tissues free from abiotic contamination. |
| 英文摘要 | Hydrastis canadensis L. (Goldenseal) is an endangered medicinal plant used in the treatment of many ailments, such as gastrointestinal disturbances, urinary disorders, hemorrhage, skin, mouth and eye infections, and inflammation. Commercial preparations of wild-harvested goldenseal were found to contain heavy metal contaminants including aluminum (848 mug g(-1)), cadmium (0.4 mug g(-1)), lead (18.7 mug g(-1)), and mercury (0.1 mug g(-1)). As well, goldenseal is an endangered species listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) Appendix II. Therefore, the practice of wild-harvest is actually decimating natural populations of goldenseal and endangering its genetic diversity. In vitro propagation protocol by tissue culture was developed for producing high-quality tissues of goldenseal. Significantly more de novo, regeneration was induced on stem explants of 3-mo.-old plants cultured on a medium containing 10 muM 6-benzylaminopurine (BA) (22 regenerants per explant) than any other treatment. Subculture of the regenerants on a medium devoid of growth regulators resulted in the development of, complete plants that were acclimatized and thrived in standard greenhouse conditions. The plants regenerated in vitro contained the lowest levels of heavy metals. The findings of this study provide the first evidence that heavy metal contaminants bioaccumulate in goldenseal tissues and also provide a method for germplasm conservation, mass multiplication, and production of goldenseal tissues free from abiotic contamination. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Plant Sciences ; Cell Biology ; Developmental Biology |
| 研究领域[WOS] | Plant Sciences ; Cell Biology ; Developmental Biology |
| 关键词[WOS] | CULTURES |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000220431300009 |
| 公开日期 | 2013-11-05 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4938]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Univ Guelph, Dept Plant Agr, Div Biotechnol, Guelph, ON N1G 2W1, Canada 2.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China 3.Natl Trop Bot Garden, Inst Ethnobot, Kalaheo, HI USA 4.Univ Notre Dame, Dept Civil Engn & Geol Sci, Notre Dame, IN 46556 USA |
| 推荐引用方式 GB/T 7714 | Liu, CZ,Murch, SJ,Jain, JC,et al. Goldenseal (Hydrastis canadensis L.): In vitro regeneration for germplasm conservation and elimination of heavy metal contamination[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,2004,40(1):75-79. |
| APA | Liu, CZ,Murch, SJ,Jain, JC,&Saxena, PK.(2004).Goldenseal (Hydrastis canadensis L.): In vitro regeneration for germplasm conservation and elimination of heavy metal contamination.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,40(1),75-79. |
| MLA | Liu, CZ,et al."Goldenseal (Hydrastis canadensis L.): In vitro regeneration for germplasm conservation and elimination of heavy metal contamination".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 40.1(2004):75-79. |
入库方式： OAI收割
来源：过程工程研究所
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