In vitro culture and temporary immersion bioreactor production of Crescentia cujete
文献类型：期刊论文
| 作者 | Murch, SJ; Liu, CZ; Romero, RM; Saxena, PK |
| 刊名 | PLANT CELL TISSUE AND ORGAN CULTURE
 |
| 出版日期 | 2004-07-01 |
| 卷号 | 78期号:1页码:63-68 |
| 关键词 | axenic cultures Calabash tree Crescentia cujete medicinal plant temporary immersion bioreactor thidiazuron |
| ISSN号 | 0167-6857 |
| 其他题名 | Plant Cell Tissue Organ Cult. |
| 中文摘要 | Crescentia cujete L. is a widely distributed medicinal tree with a diverse range of phytochemicals used as medicinal compounds. Seedlings of wild- harvested C. cujete were established in vitro and used as the starting material for the establishment of axenic cultures. Shoots were proliferated from nodal segments and were maintained over a period of more than 2 years by sequential subculture on a medium containing 1.0 mu mol l(-1) kinetin. De novo regeneration was induced on petiole sections cultured onto a medium containing thidiazuron in combination with 2,4- dichlorophenoxyacetic acid. Axenic cultures were also used to test the efficiency of three different cultivation systems for production of biomass of C. cujete. Growth of plantlets in a temporary immersion bioreactor resulted in significant increases in biomass, leaf number, shoot height and transplant efficiency. Plantlets grown in the bioreactors were acclimatized under greenhouse conditions. Together, these experiments have established optimized parameters for propagation and growth of C. cujete plantlets in a sterile controlled environment for biochemical characterization and production of high- quality medicinal products. |
| 英文摘要 | Crescentia cujete L. is a widely distributed medicinal tree with a diverse range of phytochemicals used as medicinal compounds. Seedlings of wild- harvested C. cujete were established in vitro and used as the starting material for the establishment of axenic cultures. Shoots were proliferated from nodal segments and were maintained over a period of more than 2 years by sequential subculture on a medium containing 1.0 mu mol l(-1) kinetin. De novo regeneration was induced on petiole sections cultured onto a medium containing thidiazuron in combination with 2,4- dichlorophenoxyacetic acid. Axenic cultures were also used to test the efficiency of three different cultivation systems for production of biomass of C. cujete. Growth of plantlets in a temporary immersion bioreactor resulted in significant increases in biomass, leaf number, shoot height and transplant efficiency. Plantlets grown in the bioreactors were acclimatized under greenhouse conditions. Together, these experiments have established optimized parameters for propagation and growth of C. cujete plantlets in a sterile controlled environment for biochemical characterization and production of high- quality medicinal products. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Plant Sciences |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Plant Sciences |
| 关键词[WOS] | INDUCED SOMATIC EMBRYOGENESIS ; X HORTORUM BAILEY ; THIDIAZURON ; MORPHOGENESIS ; REGULATOR ; PEANUT ; GROWTH ; FRUITS ; AUXIN |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000220303400008 |
| 公开日期 | 2013-11-05 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4951]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada 2.Natl Trop Bot Garden, Inst Ethnobot, Kalaheo, HI 96741 USA 3.Chinese Acad Sci, Inst Proc Engn, Beijing 100080, Peoples R China 4.Univ Costa Rica, Escuela Quim, San Pedro, Costa Rica 5.Inst Nacl Biodiversidad, Santo Domingo Heredia, Costa Rica |
| 推荐引用方式 GB/T 7714 | Murch, SJ,Liu, CZ,Romero, RM,et al. In vitro culture and temporary immersion bioreactor production of Crescentia cujete[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2004,78(1):63-68. |
| APA | Murch, SJ,Liu, CZ,Romero, RM,&Saxena, PK.(2004).In vitro culture and temporary immersion bioreactor production of Crescentia cujete.PLANT CELL TISSUE AND ORGAN CULTURE,78(1),63-68. |
| MLA | Murch, SJ,et al."In vitro culture and temporary immersion bioreactor production of Crescentia cujete".PLANT CELL TISSUE AND ORGAN CULTURE 78.1(2004):63-68. |
入库方式： OAI收割
来源：过程工程研究所
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