In vitro protein refolding by chromatographic procedures
文献类型：期刊论文
| 作者 | Li, M; Su, ZG; Janson, JC |
| 刊名 | PROTEIN EXPRESSION AND PURIFICATION
 |
| 出版日期 | 2004 |
| 卷号 | 33期号:1页码:1-10 |
| 关键词 | in vitro protein refolding inclusion bodies chromatographic techniques gradient techniques |
| ISSN号 | 1046-5928 |
| 其他题名 | Protein Expr. Purif. |
| 中文摘要 | In vitro protein refolding is still a bottleneck in both structural biology and in the development of new biopharmaceuticals, especially for commercially important polypeptides that are overexpressed in Escherichia coli. This review focuses on protein refolding methods based on column procedures because recent advances in chromatographic refolding have shown promising results. (C) 2003 Elsevier Inc. All rights reserved. |
| 英文摘要 | In vitro protein refolding is still a bottleneck in both structural biology and in the development of new biopharmaceuticals, especially for commercially important polypeptides that are overexpressed in Escherichia coli. This review focuses on protein refolding methods based on column procedures because recent advances in chromatographic refolding have shown promising results. (C) 2003 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | SIZE-EXCLUSION CHROMATOGRAPHY ; ION-EXCHANGE CHROMATOGRAPHY ; IMMOBILIZED LIPOSOME CHROMATOGRAPHY ; UROKINASE-PLASMINOGEN-ACTIVATOR ; COLI INCLUSION-BODIES ; EGG-WHITE LYSOZYME ; ESCHERICHIA-COLI ; RECOMBINANT PROTEINS ; CARBONIC-ANHYDRASE ; STEP PURIFICATION |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000187587500001 |
| 公开日期 | 2013-11-05 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4952]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Uppsala Biomed Ctr, Dept Surface Biotechnol, SE-75123 Uppsala, Sweden 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China 3.Amersham Biosci R&D Dept, Uppsala, Sweden |
| 推荐引用方式 GB/T 7714 | Li, M,Su, ZG,Janson, JC. In vitro protein refolding by chromatographic procedures[J]. PROTEIN EXPRESSION AND PURIFICATION,2004,33(1):1-10. |
| APA | Li, M,Su, ZG,&Janson, JC.(2004).In vitro protein refolding by chromatographic procedures.PROTEIN EXPRESSION AND PURIFICATION,33(1),1-10. |
| MLA | Li, M,et al."In vitro protein refolding by chromatographic procedures".PROTEIN EXPRESSION AND PURIFICATION 33.1(2004):1-10. |
入库方式： OAI收割
来源：过程工程研究所
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