Microencapsulation of bovine hemoglobin with high bio-activity and high entrapment efficiency using a W/O/W double emulsion technique
文献类型：期刊论文
| 作者 | Meng, FT; Ma, GH; Liu, YD; Qiu, W; Su, ZG |
| 刊名 | COLLOIDS AND SURFACES B-BIOINTERFACES
 |
| 出版日期 | 2004-02-15 |
| 卷号 | 33期号:3-4页码:177-183 |
| 关键词 | monomethoxypoly(ethylene glycol)-b-poly-D biodegradable microspheres bovine hemoglobin stability protein entrapment efficiency L-lactide |
| ISSN号 | 0927-7765 |
| 其他题名 | Colloid Surf. B-Biointerfaces |
| 中文摘要 | This study focused on the influences of solvent removal method and wall polymer composition on microspheres characteristics in W/O/W double emulsion procedure. Monomethoxypoly(ethylene glycol)-b-poly-D,L-lactide (PELA) microspheres containing bovine hemoglobin (BHb, a model protein) were prepared by four solvent removal methods, including solvent-evaporation at atmosphere, at reduced pressure, solvent-extraction and solvent-diffusion methods, where the last method used ethyl acetate (EA) as organic solvent and the others used methylene chloride (MC). The bio-activity of encapsulated BHb, encapsulation efficiency, particle size and surface morphology of microspheres were evaluated in relation to the influences of solvent removal method and PELA composition. BHb encapsulated by the W/O/W double emulsion-sol vent diffusion method with EA as organic solvent displayed a bio-activity near to that of native BHb. The efficiency of BHb entrapment achieved by this method was much higher than those by other methods (ca. 90% versus 30%). When using this process, the copolymers with MPEG 2000 block (molecular weight of PEG block: 2000 g/mol) yielded much higher efficiencies of BHb entrapment than those with MPEG 5000 block (90% versus 36%). Copolymer composition had less impact on microsphere size, but had a pronounced effect on surface morphology of microspheres. This study suggests that the W/O/W double emulsion-solvent diffusion method with EA as organic solvent is an effective process to prepare microspheres containing therapeutic proteins, and that the PELA copolymers containing MPEG 2000 block are promising wall material for biodegradable microsphere protein delivery system. (C) 2003 Elsevier B.V. All rights reserved. |
| 英文摘要 | This study focused on the influences of solvent removal method and wall polymer composition on microspheres characteristics in W/O/W double emulsion procedure. Monomethoxypoly(ethylene glycol)-b-poly-D,L-lactide (PELA) microspheres containing bovine hemoglobin (BHb, a model protein) were prepared by four solvent removal methods, including solvent-evaporation at atmosphere, at reduced pressure, solvent-extraction and solvent-diffusion methods, where the last method used ethyl acetate (EA) as organic solvent and the others used methylene chloride (MC). The bio-activity of encapsulated BHb, encapsulation efficiency, particle size and surface morphology of microspheres were evaluated in relation to the influences of solvent removal method and PELA composition. BHb encapsulated by the W/O/W double emulsion-sol vent diffusion method with EA as organic solvent displayed a bio-activity near to that of native BHb. The efficiency of BHb entrapment achieved by this method was much higher than those by other methods (ca. 90% versus 30%). When using this process, the copolymers with MPEG 2000 block (molecular weight of PEG block: 2000 g/mol) yielded much higher efficiencies of BHb entrapment than those with MPEG 5000 block (90% versus 36%). Copolymer composition had less impact on microsphere size, but had a pronounced effect on surface morphology of microspheres. This study suggests that the W/O/W double emulsion-solvent diffusion method with EA as organic solvent is an effective process to prepare microspheres containing therapeutic proteins, and that the PELA copolymers containing MPEG 2000 block are promising wall material for biodegradable microsphere protein delivery system. (C) 2003 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology |
| 类目[WOS] | Biophysics ; Chemistry, Physical ; Materials Science, Biomaterials |
| 研究领域[WOS] | Biophysics ; Chemistry ; Materials Science |
| 关键词[WOS] | BIODEGRADABLE MICROSPHERES ; GLYCOL) MICROSPHERES ; ENCAPSULATION ; LYSOZYME ; PROTEIN ; MICROPARTICLES ; INTERFACE ; STABILITY ; CHLORIDE |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000189085900005 |
| 公开日期 | 2013-11-05 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4960]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Meng, FT,Ma, GH,Liu, YD,et al. Microencapsulation of bovine hemoglobin with high bio-activity and high entrapment efficiency using a W/O/W double emulsion technique[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2004,33(3-4):177-183. |
| APA | Meng, FT,Ma, GH,Liu, YD,Qiu, W,&Su, ZG.(2004).Microencapsulation of bovine hemoglobin with high bio-activity and high entrapment efficiency using a W/O/W double emulsion technique.COLLOIDS AND SURFACES B-BIOINTERFACES,33(3-4),177-183. |
| MLA | Meng, FT,et al."Microencapsulation of bovine hemoglobin with high bio-activity and high entrapment efficiency using a W/O/W double emulsion technique".COLLOIDS AND SURFACES B-BIOINTERFACES 33.3-4(2004):177-183. |
入库方式： OAI收割
来源：过程工程研究所
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