Partial purification of alpha-amylase from culture supernatant of Bacillus subtilis in aqueous two-phase systems
文献类型：期刊论文
| 作者 | Zhi, WB; Song, JM; Bi, JX; Fan, OY |
| 刊名 | BIOPROCESS AND BIOSYSTEMS ENGINEERING
 |
| 出版日期 | 2004-12-01 |
| 卷号 | 27期号:1页码:3-7 |
| 关键词 | alpha-amylase aqueous two-phase systems purification |
| ISSN号 | 1615-7591 |
| 其他题名 | Bioprocess. Biosyst. Eng. |
| 中文摘要 | A study was made of the partition and purification of alpha-amylase from a culture supernatant of Bacillus subtilis in the polyethylene glycol (PEG)-citrate aqueous two-phase system (ATPS). Factors that influenced the partition of the protein in this system, including the molecular weight of the PEG, the tie line length of ATPS, the pH value and the sodium chloride concentration, were investigated. Purification of alpha-amylase was attained with a purification factor (PF) of 1.8 and 90% yield at pH 6.0 in a PEG1000-citrate ATPS with short tie line length. By utilizing the salt-out effect of neutral salt, the purification of alpha-amylase was further improved to 2.0 of PF and 80% yield in a PEG3350-citrate ATPS with 4% sodium chloride. |
| 英文摘要 | A study was made of the partition and purification of alpha-amylase from a culture supernatant of Bacillus subtilis in the polyethylene glycol (PEG)-citrate aqueous two-phase system (ATPS). Factors that influenced the partition of the protein in this system, including the molecular weight of the PEG, the tie line length of ATPS, the pH value and the sodium chloride concentration, were investigated. Purification of alpha-amylase was attained with a purification factor (PF) of 1.8 and 90% yield at pH 6.0 in a PEG1000-citrate ATPS with short tie line length. By utilizing the salt-out effect of neutral salt, the purification of alpha-amylase was further improved to 2.0 of PF and 80% yield in a PEG3350-citrate ATPS with 4% sodium chloride. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | PARTITION |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000226236000002 |
| 公开日期 | 2013-11-05 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4983]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhi, WB,Song, JM,Bi, JX,et al. Partial purification of alpha-amylase from culture supernatant of Bacillus subtilis in aqueous two-phase systems[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2004,27(1):3-7. |
| APA | Zhi, WB,Song, JM,Bi, JX,&Fan, OY.(2004).Partial purification of alpha-amylase from culture supernatant of Bacillus subtilis in aqueous two-phase systems.BIOPROCESS AND BIOSYSTEMS ENGINEERING,27(1),3-7. |
| MLA | Zhi, WB,et al."Partial purification of alpha-amylase from culture supernatant of Bacillus subtilis in aqueous two-phase systems".BIOPROCESS AND BIOSYSTEMS ENGINEERING 27.1(2004):3-7. |
入库方式： OAI收割
来源：过程工程研究所
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