Reverse micelles extraction of nattokinase from fermentation broth
文献类型：期刊论文
| 作者 | Liu, JG; Xing, JM; Shen, R; Yang, CL; Liu, HZ |
| 刊名 | BIOCHEMICAL ENGINEERING JOURNAL
 |
| 出版日期 | 2004-11-01 |
| 卷号 | 21期号:3页码:273-278 |
| 关键词 | reverse micelles nattokinase purification AOT protein purification |
| ISSN号 | 1369-703X |
| 其他题名 | Biochem. Eng. J. |
| 中文摘要 | Nattokinase is a new fibrinolytic enzyme, which cleaves directly cross-linked fibrin in vitro. As its name implies, it is produced in the natto fermentation process. In this study, the purification of nattokinase from fermentation broth with AOT/isooctane micellar solution as the extractant was studied. Many factors, including pH, surfactant concentration, addition of isopropanol and contact time, were examined. By using the reversed micelles of AOT/isooctane to perform a full extraction cycle, it was found that about 80% of the total activity of nattokinase in the fermentation broth could be recovered and the purification factor was about 2.7. (C) 2004 Elsevier B.V. All rights reserved. |
| 英文摘要 | Nattokinase is a new fibrinolytic enzyme, which cleaves directly cross-linked fibrin in vitro. As its name implies, it is produced in the natto fermentation process. In this study, the purification of nattokinase from fermentation broth with AOT/isooctane micellar solution as the extractant was studied. Many factors, including pH, surfactant concentration, addition of isopropanol and contact time, were examined. By using the reversed micelles of AOT/isooctane to perform a full extraction cycle, it was found that about 80% of the total activity of nattokinase in the fermentation broth could be recovered and the purification factor was about 2.7. (C) 2004 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | FIBRINOLYTIC ENZYME NATTOKINASE ; VEGETABLE CHEESE NATTO ; ALPHA-CHYMOTRYPSIN ; ORGANIC MEDIA ; PROTEINS ; TRANSPORT ; PEPSIN ; STATE ; FOOD ; RAT |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000224295400011 |
| 公开日期 | 2013-11-05 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5010]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Lab Separat Sci & Engn, Inst Proc Engn, Beijing 100080, Peoples R China 2.Hebei Univ Sci & Technol, Coll Biosci & Bioengn, Shijiazhuang 050018, Hebei Province, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, JG,Xing, JM,Shen, R,et al. Reverse micelles extraction of nattokinase from fermentation broth[J]. BIOCHEMICAL ENGINEERING JOURNAL,2004,21(3):273-278. |
| APA | Liu, JG,Xing, JM,Shen, R,Yang, CL,&Liu, HZ.(2004).Reverse micelles extraction of nattokinase from fermentation broth.BIOCHEMICAL ENGINEERING JOURNAL,21(3),273-278. |
| MLA | Liu, JG,et al."Reverse micelles extraction of nattokinase from fermentation broth".BIOCHEMICAL ENGINEERING JOURNAL 21.3(2004):273-278. |
入库方式： OAI收割
来源：过程工程研究所
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