Simulation of size exclusion chromatography for characterization of supramolecular complex: a theoretical study
文献类型：期刊论文
| 作者 | Lou, XW; Zhu, QS; Lei, Z; van Dongen, JLJ; Meijer, EW |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2004-03-12 |
| 卷号 | 1029期号:1-2页码:67-75 |
| 关键词 | simulation supramolecular complex retention behavior association constant size exclusion chromatography |
| ISSN号 | 0021-9673 |
| 其他题名 | J. Chromatogr. A |
| 中文摘要 | A simple chromatographic model was used to simulate the retention behavior of supramolecular complex in size exclusion chromatography (SEC). Two fundamental model complexes, directional complementary couple (AB) and self-complementary unit (AA), were employed to investigate the influence of various parameters in the SEC characterization/confirmation of supramolecular association. Peak tailing, peak fronting, peak splitting and retention time shifting were observed under different conditions. It was found that the chromatographic peak shape and retention time were strongly affected by the association constant, sample concentration, as well as the addition of a reactant in the mobile phase. Furthermore, using the same model, the chromatographic process of Hummel-Dreyer procedure was simulated, and the results indicated that the procedure can be a good method for the determination of association constants for AB type complexes. (C) 2004 Elsevier B.V. All rights reserved. |
| 英文摘要 | A simple chromatographic model was used to simulate the retention behavior of supramolecular complex in size exclusion chromatography (SEC). Two fundamental model complexes, directional complementary couple (AB) and self-complementary unit (AA), were employed to investigate the influence of various parameters in the SEC characterization/confirmation of supramolecular association. Peak tailing, peak fronting, peak splitting and retention time shifting were observed under different conditions. It was found that the chromatographic peak shape and retention time were strongly affected by the association constant, sample concentration, as well as the addition of a reactant in the mobile phase. Furthermore, using the same model, the chromatographic process of Hummel-Dreyer procedure was simulated, and the results indicated that the procedure can be a good method for the determination of association constants for AB type complexes. (C) 2004 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | PROTEIN-BINDING ; GEL-FILTRATION ; SMALL-ZONE |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000189076000009 |
| 公开日期 | 2013-11-05 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5017]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Tech Univ Eindhoven, Lab Macromol & Organ Chem, NL-5600 MB Eindhoven, Netherlands 2.Chinese Acad Sci, Inst Proc Engn, Multiphase React Lab, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lou, XW,Zhu, QS,Lei, Z,et al. Simulation of size exclusion chromatography for characterization of supramolecular complex: a theoretical study[J]. JOURNAL OF CHROMATOGRAPHY A,2004,1029(1-2):67-75. |
| APA | Lou, XW,Zhu, QS,Lei, Z,van Dongen, JLJ,&Meijer, EW.(2004).Simulation of size exclusion chromatography for characterization of supramolecular complex: a theoretical study.JOURNAL OF CHROMATOGRAPHY A,1029(1-2),67-75. |
| MLA | Lou, XW,et al."Simulation of size exclusion chromatography for characterization of supramolecular complex: a theoretical study".JOURNAL OF CHROMATOGRAPHY A 1029.1-2(2004):67-75. |
入库方式： OAI收割
来源：过程工程研究所
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