Synthesis of amino-silane modified superparamagnetic silica supports and their use for protein immobilization
文献类型：期刊论文
| 作者 | Liu, XQ; Xing, JM; Guan, YP; Shan, GB; Liu, HZ |
| 刊名 | COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
 |
| 出版日期 | 2004-05-04 |
| 卷号 | 238期号:1-3页码:127-131 |
| 关键词 | magnetic supports amino-silane protein immobilization bioseparation |
| ISSN号 | 0927-7757 |
| 其他题名 | Colloid Surf. A-Physicochem. Eng. Asp. |
| 中文摘要 | A novel method to synthesize amino-silane modified magnetic silica supports was reported for affinity separation. The magnetite (Fe3O4) nanoparticles were prepared by the coprecipitation of ferrous and ferric salts with NH4OH, and then their surface was coated with silica in sodium silicate solution by acidifying technology to form well-dispersed magnetic silica nanospheres. These magnetic silica nanospheres are of about 50-80 nm in diameter and exhibit superparamagnetic characteristics. The surface of these nanospheres was with amino-silane coupling agent for their attachment to affinity ligands. In this work, bovine serum albumin (BSA) was covalently immobilized onto the amino-silane modified magnetic silica supports by the glutaraldehyde method. The influence of pH, ionic strength as well as the initial protein concentration on BSA immobilization has been studied. The result shows that such amino-silane modified magnetic silica is a well-dispersed and effective superparamagnetic support for bioseparation and the maximum BSA immobilization capacity (up to 86 mg/g) was obtained in 0.1 M phosphate buffer at pH 5.0. (C) 2004 Elsevier B.V. All rights reserved. |
| 英文摘要 | A novel method to synthesize amino-silane modified magnetic silica supports was reported for affinity separation. The magnetite (Fe3O4) nanoparticles were prepared by the coprecipitation of ferrous and ferric salts with NH4OH, and then their surface was coated with silica in sodium silicate solution by acidifying technology to form well-dispersed magnetic silica nanospheres. These magnetic silica nanospheres are of about 50-80 nm in diameter and exhibit superparamagnetic characteristics. The surface of these nanospheres was with amino-silane coupling agent for their attachment to affinity ligands. In this work, bovine serum albumin (BSA) was covalently immobilized onto the amino-silane modified magnetic silica supports by the glutaraldehyde method. The influence of pH, ionic strength as well as the initial protein concentration on BSA immobilization has been studied. The result shows that such amino-silane modified magnetic silica is a well-dispersed and effective superparamagnetic support for bioseparation and the maximum BSA immobilization capacity (up to 86 mg/g) was obtained in 0.1 M phosphate buffer at pH 5.0. (C) 2004 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Physical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | FINE MAGNETIC PARTICLES ; MICROEMULSION ; PURIFICATION ; MICROSPHERES ; ADSORPTION ; ENZYMES |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000222077400016 |
| 公开日期 | 2013-11-05 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5029]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Lab Separat Sci & Engn, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, XQ,Xing, JM,Guan, YP,et al. Synthesis of amino-silane modified superparamagnetic silica supports and their use for protein immobilization[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2004,238(1-3):127-131. |
| APA | Liu, XQ,Xing, JM,Guan, YP,Shan, GB,&Liu, HZ.(2004).Synthesis of amino-silane modified superparamagnetic silica supports and their use for protein immobilization.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,238(1-3),127-131. |
| MLA | Liu, XQ,et al."Synthesis of amino-silane modified superparamagnetic silica supports and their use for protein immobilization".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 238.1-3(2004):127-131. |
入库方式： OAI收割
来源：过程工程研究所
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