胸腺素α_1的DIC固相化学合成与鉴定
文献类型：期刊论文
| 作者 | 甘一如 ; 黄永东 ; 刘晋生 ; 张雪竹 ; 张贵峰 |
| 刊名 | 化工学报
 |
| 出版日期 | 2004 |
| 期号 | 02页码:305-308 |
| 关键词 | 胸腺素α1 固相多肽合成 二异丙基碳二亚胺(DIC) 反相高效液相色谱法 聚丙烯酰胺凝胶电泳(SDSPAGE)法 液相色谱/质谱(LC/MS) |
| 中文摘要 | Thymosin α 1 was synthesized manually. The washing condition and the test of the degree of coupling were studied. In addition, the analytical method of crude product was set up. Thymosin α 1 was synthesized with the solid-phase method, by using the base-liable Fmoc group to protect the α-amino acid and N,N′-diisopropylcarbodiimide (DIC) as the coupling regent. The crude product was analyzed by reversed-phase high performance liquid chromatography, SDS-PAGE and liquid chromatography/mass spectroscopy.The synthesis yield was 84.89%. The analytical results indicated that the crude product contained much thymosin α 1, and the purity was 50.3%. DIC solid-phase chemical synthesis of thymosin α 1 was applicable, and the yield was improved greatly compared to other known methods. |
| 公开日期 | 2013-11-07 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5218]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | 甘一如,黄永东,刘晋生,等. 胸腺素α_1的DIC固相化学合成与鉴定[J]. 化工学报,2004(02):305-308. |
| APA | 甘一如,黄永东,刘晋生,张雪竹,&张贵峰.(2004).胸腺素α_1的DIC固相化学合成与鉴定.化工学报(02),305-308. |
| MLA | 甘一如,et al."胸腺素α_1的DIC固相化学合成与鉴定".化工学报 .02(2004):305-308. |
入库方式： OAI收割
来源：过程工程研究所
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