The preparation and characterization of monomethoxypoly(ethylene glycol)b-poly-DL-lactide microcapsules containing bovine hemoglobin
文献类型：期刊论文
| 作者 | Meng, FT; Zhang, WZ; Ma, GH; Su, ZG |
| 刊名 | ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY
 |
| 出版日期 | 2003 |
| 卷号 | 31期号:3页码:279-292 |
| 关键词 | blood substitute bovine hemoglobin microcapsule monomethoxypoly(ethylene glycol)-b-poly-DL-lactide ethyl acetate |
| ISSN号 | 1073-1199 |
| 其他题名 | Artif. Cells Blood Substit. Immobil. Biotechnol. |
| 中文摘要 | Methoxypoly(ethylene glycol)-b-poly-DL-lactide (PELA) microcapsules containing bovine hemoglobin (bHb) were prepared by a W/O/W double emulsion-solvent diffusion process. bHb solution was used as the internal aqueous phase, PELA/organic solvent as the oil phase, and polyvinyl alcohol (PVA) solution as the external aqueous phase. This W/O/W double emulsion was added into a large volume of water (solidification solution) to allow organic solvent to diffuse into water. The optimum preparative condition for PELA microcapsules loaded with bovine hemoglobin was investigated. It was found that homogenization rate, type of organic solvent, and volume of the solidification solution influenced the activity of bovine hemoglobin encapsulated. When the homogenization rate was lower than 9000 rpm and ethyl acetate was used as the organic solvent, there was no significant influence on the activity of hemoglobin. High homogenization rate as 12 000 rpm decreased the P-50 and Hill coefficient. Increasing the volume of solidification solution had an effect of improving the activity of microencapsulated hemoglobin. The composition of the PELA had the most important influence on the success of encapsulation. Microcapsules fabricated by PELA with MPEG2k block (molecular weight of MPEG block: 2000) achieved a high entrapment efficiency of 90%, better than PLA homopolymer and PELA with MPEG5k blocks. Hemoglobin microcapsules with native loading oxygen activity (P-50 = 26.0 mmHg, Hill coefficient = 2.4), mean size of about 10 pm, and high entrapment efficiency (ca. 93%) were obtained at the optimum condition. |
| 英文摘要 | Methoxypoly(ethylene glycol)-b-poly-DL-lactide (PELA) microcapsules containing bovine hemoglobin (bHb) were prepared by a W/O/W double emulsion-solvent diffusion process. bHb solution was used as the internal aqueous phase, PELA/organic solvent as the oil phase, and polyvinyl alcohol (PVA) solution as the external aqueous phase. This W/O/W double emulsion was added into a large volume of water (solidification solution) to allow organic solvent to diffuse into water. The optimum preparative condition for PELA microcapsules loaded with bovine hemoglobin was investigated. It was found that homogenization rate, type of organic solvent, and volume of the solidification solution influenced the activity of bovine hemoglobin encapsulated. When the homogenization rate was lower than 9000 rpm and ethyl acetate was used as the organic solvent, there was no significant influence on the activity of hemoglobin. High homogenization rate as 12 000 rpm decreased the P-50 and Hill coefficient. Increasing the volume of solidification solution had an effect of improving the activity of microencapsulated hemoglobin. The composition of the PELA had the most important influence on the success of encapsulation. Microcapsules fabricated by PELA with MPEG2k block (molecular weight of MPEG block: 2000) achieved a high entrapment efficiency of 90%, better than PLA homopolymer and PELA with MPEG5k blocks. Hemoglobin microcapsules with native loading oxygen activity (P-50 = 26.0 mmHg, Hill coefficient = 2.4), mean size of about 10 pm, and high entrapment efficiency (ca. 93%) were obtained at the optimum condition. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Biomedical ; Materials Science, Biomaterials |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering ; Materials Science |
| 关键词[WOS] | POTENTIAL BLOOD SUBSTITUTES ; DOUBLE EMULSION TECHNIQUE ; MICROSPHERES ; PROTEIN ; NANOCAPSULES |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000184447900004 |
| 公开日期 | 2013-11-08 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5328]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Shenyang Inst Chem Technol, Dept Chem Engn, Shenyang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Meng, FT,Zhang, WZ,Ma, GH,et al. The preparation and characterization of monomethoxypoly(ethylene glycol)b-poly-DL-lactide microcapsules containing bovine hemoglobin[J]. ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY,2003,31(3):279-292. |
| APA | Meng, FT,Zhang, WZ,Ma, GH,&Su, ZG.(2003).The preparation and characterization of monomethoxypoly(ethylene glycol)b-poly-DL-lactide microcapsules containing bovine hemoglobin.ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY,31(3),279-292. |
| MLA | Meng, FT,et al."The preparation and characterization of monomethoxypoly(ethylene glycol)b-poly-DL-lactide microcapsules containing bovine hemoglobin".ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY 31.3(2003):279-292. |
入库方式： OAI收割
来源：过程工程研究所
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