The preparation of an artificial red blood cell substitute by W/O/W double emulsion methods
文献类型：期刊论文
| 作者 | Qiu, W; Meng, FT; Ma, GH; Su, ZG |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2003-05-01 |
| 卷号 | 35期号:5页码:467-472 |
| 关键词 | artificial red cells methoxypoly(ethylene glycol)-poly-DL-lactide bovine hemoglobin microcapsule |
| ISSN号 | 0582-9879 |
| 其他题名 | Acta Biochim. Biophys. Sin. |
| 中文摘要 | A method of preparing methoxypoly (ethylene glycol)-poly-DL-lactide (PELA) microcapsule containing bovine hemoglobin(BHb) by using W/O/W double emulsion-solvent diffusion process for use as artificial red cells was developed. The results suggested that preparation conditions such as solidification method, stirring rate, type of organic solvent had significant influence on the encapsulation efficiency and the activity of bovine hemoglobin. When ethyl acetate was used as the organic solvent and double emulsion-solvent diffusion process was used, there was no significant influence on the activity of hemoglobin if stirring rate was lower than 9000 r/min. High stirring rate, 12 000 r/min, decreased the P-50 and Hill coefficient of the hemoglobin encapsulated. Increasing the volume of solidification solution had an effect of improving the activity of hemoglobin to carry oxygen. |
| 英文摘要 | A method of preparing methoxypoly (ethylene glycol)-poly-DL-lactide (PELA) microcapsule containing bovine hemoglobin(BHb) by using W/O/W double emulsion-solvent diffusion process for use as artificial red cells was developed. The results suggested that preparation conditions such as solidification method, stirring rate, type of organic solvent had significant influence on the encapsulation efficiency and the activity of bovine hemoglobin. When ethyl acetate was used as the organic solvent and double emulsion-solvent diffusion process was used, there was no significant influence on the activity of hemoglobin if stirring rate was lower than 9000 r/min. High stirring rate, 12 000 r/min, decreased the P-50 and Hill coefficient of the hemoglobin encapsulated. Increasing the volume of solidification solution had an effect of improving the activity of hemoglobin to carry oxygen. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | MEMBRANE HEMOGLOBIN NANOCAPSULES ; POLY(LACTIC ACID) ; MICROSPHERES ; PROTEIN ; ENCAPSULATION ; PEG |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000183076800012 |
| 公开日期 | 2013-11-08 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5332]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiu, W,Meng, FT,Ma, GH,et al. The preparation of an artificial red blood cell substitute by W/O/W double emulsion methods[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(5):467-472. |
| APA | Qiu, W,Meng, FT,Ma, GH,&Su, ZG.(2003).The preparation of an artificial red blood cell substitute by W/O/W double emulsion methods.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(5),467-472. |
| MLA | Qiu, W,et al."The preparation of an artificial red blood cell substitute by W/O/W double emulsion methods".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.5(2003):467-472. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。