Separation and identification of different refolding components
文献类型：期刊论文
| 作者 | Li, M; Su, ZG |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2003-06-26 |
| 卷号 | 103期号:2页码:119-127 |
| 关键词 | size-exclusion chromatography ion-exchange chromatography hydrophobic interaction chromatography refolding aggregation |
| ISSN号 | 0168-1656 |
| 其他题名 | J. Biotechnol. |
| 中文摘要 | A dual-gradient ion-exchange chromatography (IEC) refolding process has been successfully applied to renature lysozyme at high concentration. The different refolding components were separated by size-exclusion chromatography with low concentrations of urea and NaCl added into elution buffer. The hydrodynamic characteristics during separation and the hydrophobic properties of these components were also tested. (C) 2003 Elsevier Science B.V. All rights reserved. |
| 英文摘要 | A dual-gradient ion-exchange chromatography (IEC) refolding process has been successfully applied to renature lysozyme at high concentration. The different refolding components were separated by size-exclusion chromatography with low concentrations of urea and NaCl added into elution buffer. The hydrodynamic characteristics during separation and the hydrophobic properties of these components were also tested. (C) 2003 Elsevier Science B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | SIZE-EXCLUSION CHROMATOGRAPHY ; HUMAN INTERFERON-GAMMA ; DENATURED/REDUCED LYSOZYME ; DISEASE ; RENATURATION ; AGGREGATION ; ALZHEIMERS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000183825000004 |
| 公开日期 | 2013-11-08 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5344]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, M,Su, ZG. Separation and identification of different refolding components[J]. JOURNAL OF BIOTECHNOLOGY,2003,103(2):119-127. |
| APA | Li, M,&Su, ZG.(2003).Separation and identification of different refolding components.JOURNAL OF BIOTECHNOLOGY,103(2),119-127. |
| MLA | Li, M,et al."Separation and identification of different refolding components".JOURNAL OF BIOTECHNOLOGY 103.2(2003):119-127. |
入库方式： OAI收割
来源：过程工程研究所
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