A solid phase adsorption method for preparation of bovine serum albumin-bovine hemoglobin conjugate
文献类型：期刊论文
| 作者 | Hu, T; Su, ZG |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2003-02-13 |
| 卷号 | 100期号:3页码:267-275 |
| 关键词 | blood substitute bovine hemoglobin bovine serum albumin glutaraldehyde solid phase adsorption |
| ISSN号 | 0168-1656 |
| 其他题名 | J. Biotechnol. |
| 中文摘要 | A solid phase adsorption method was proposed to prepare well-defined bovine serum albumin-bovine hemoglobin (Hb) conjugate. After adsorption by the solid phase, Q Sepharose Fast Flow media, bovine serum albumin (BSA) molecules were allowed to react with glutaraldehyde. The spacing out of BSA molecules on the solid phase was assumed to limit polymerization of BSA molecules, except some molecules bound closely on the solid phase resulting in minor dimer formation. Following the elution procedure, the activated monomeric BSA was separated from the dimers by gel filtration chromatography on Superdex 200 and then reacted with bovine Hb at 4 degreesC and pH 9.5. The 1:1 (BSA:Hb) conjugate was obtained with the yield of 64%. The P-50 values of the conjugates, prepared under anaerobic and aerobic conditions, were 19.1 and 14.2 mmHg, respectively. The dependence of the P-50 on chloride ions for the conjugate was slightly diminished, presumably due to covalent attachment of BSA to bovine Hb. (C) 2002 Elsevier Science B.V. All rights reserved. |
| 英文摘要 | A solid phase adsorption method was proposed to prepare well-defined bovine serum albumin-bovine hemoglobin (Hb) conjugate. After adsorption by the solid phase, Q Sepharose Fast Flow media, bovine serum albumin (BSA) molecules were allowed to react with glutaraldehyde. The spacing out of BSA molecules on the solid phase was assumed to limit polymerization of BSA molecules, except some molecules bound closely on the solid phase resulting in minor dimer formation. Following the elution procedure, the activated monomeric BSA was separated from the dimers by gel filtration chromatography on Superdex 200 and then reacted with bovine Hb at 4 degreesC and pH 9.5. The 1:1 (BSA:Hb) conjugate was obtained with the yield of 64%. The P-50 values of the conjugates, prepared under anaerobic and aerobic conditions, were 19.1 and 14.2 mmHg, respectively. The dependence of the P-50 on chloride ions for the conjugate was slightly diminished, presumably due to covalent attachment of BSA to bovine Hb. (C) 2002 Elsevier Science B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | OXYGEN |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000180578500010 |
| 公开日期 | 2013-11-08 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5350]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, Natl Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, T,Su, ZG. A solid phase adsorption method for preparation of bovine serum albumin-bovine hemoglobin conjugate[J]. JOURNAL OF BIOTECHNOLOGY,2003,100(3):267-275. |
| APA | Hu, T,&Su, ZG.(2003).A solid phase adsorption method for preparation of bovine serum albumin-bovine hemoglobin conjugate.JOURNAL OF BIOTECHNOLOGY,100(3),267-275. |
| MLA | Hu, T,et al."A solid phase adsorption method for preparation of bovine serum albumin-bovine hemoglobin conjugate".JOURNAL OF BIOTECHNOLOGY 100.3(2003):267-275. |
入库方式： OAI收割
来源：过程工程研究所
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